PXD005246

PXD005246 is an original dataset announced via ProteomeXchange.

Title	SILAC phosphoproteomics of 293-F10 cells [induced (H) vs uninduced (L)]
Description	The objective of this experiment was to identify cellular targets of F10-mediated phosphorylation through Stable Isotope Labeling of Amino acids in Cell culture (SILAC). 293-F10 cells were propagated in heavy (H) or light (L) media for 10 cellular doublings. Mass spectrometry was used to confirm that the majority of tryptic peptides showed >90% incorporation of the heavy isotope. H-labeled cells were treated with doxycycline to induce expression of F10 for 10 hours, and then these cells were mixed with uninduced, L-labeled cells in a 1:1 ratio. Three biological replicates were obtained from separately passaged cultures and doxycycline inductions. Cells were lysed and post-nuclear supernatant proteins were precipitated and subjected to tryptic digestion. Tryptic peptides were desalted, enriched for phosphopeptides, and analyzed in triplicate by LC-MS/MS. The resulting 9 data files were pooled and phosphoprotein identification and quantification was completed using MaxQuant software (version 1.2.2.5). Differential phosphorylation in response to F10 induction was determined using an outlier score for log protein ratios. The Benjamini-Hochberg test was then used to correct for the biased statistical spread of highly abundant proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:35:13.726.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michaela Pereckas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-01 03:06:11	ID requested
1	2017-02-15 07:50:46	announced
⏵ 2	2024-10-22 04:35:19	announced	2024-10-22: Updated project metadata.

10.1074/mcp.M116.065003;

Greseth MD, Carter DC, Terhune SS, Traktman P, Proteomic Screen for Cellular Targets of the Vaccinia Virus F10 Protein Kinase Reveals that Phosphorylation of mDia Regulates Stress Fiber Formation. Mol Cell Proteomics, 16(4 suppl 1):S124-S143(2017) [pubmed]

curator keyword: Biological

submitter keyword: F10 protein kinase, Vaccinia Virus,SILAC, Phosphoproteome

Paula Traktman
contact affiliation	Departments of Biochemistry & Molecular Biology and Microbiology & Immunology Medical University of South Carolina USA
contact email	traktman@musc.edu
lab head
Michaela Pereckas
contact affiliation	Medical College of Wisconsin
contact email	msp@mcw.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD005246

PRIDE project URI

• PRIDE
  1. PXD005246
     1. Label: PRIDE project
     2. Name: SILAC phosphoproteomics of 293-F10 cells [induced (H) vs uninduced (L)]