PXD005234 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | In vivo and in vitro proteome analysis of HIV-1 infected, human CD4+ T cells |
Description | Host directed therapies against HIV-1 are thought to be critical for long term containment of the HIV-1 pandemic but remain elusive. Since HIV-1 infects and manipulates important effectors of both the innate and adaptive immune system, identifying modulations of the host cell systems in humans during HIV-1 infection may be crucial for the development of immune based therapies. Here, we quantified the changes of the proteome in human CD4+ T cells upon HIV-1 infection, both in vitro and in vivo. A SWATH-MS approach was used to measure the proteome of human primary CD4+ T cells infected with HIV-1 in vitro as well as CD4+ T cells from HIV-1 infected patients with paired samples on and off antiretroviral treatment. In the in vitro experiment, the proteome of CD4+ T cells was quantified over a time course following HIV-1 infection. 1,725 host cell proteins and 4 HIV-1 proteins were quantified, with 145 proteins changing significantly during the time course. Changes in the proteome peaked 24 hours after infection, concomitantly with significant HIV-1 protein production. In the in vivo branch of the study, CD4+ T cells from viremic patients and those with no detectable viral load after treatment were sorted and the proteomes quantified. We consistently detected 895 proteins, 172 of which were considered to be significantly different between viraemic patients and patients undergoing successful treatment. The proteome of in vitro infected CD4+ T cells was modulated on multiple functional levels, including TLR-4 signalling and the type 1 interferon signalling pathway. Perturbations in the type 1 interferon signalling pathway were recapitulated in CD4+ T cells from patients. The study shows that proteome maps generated by SWATH-MS indicate a range of functionally significant changes in the proteome of HIV infected human CD4+ T cells. Exploring these perturbations in more detail may help identify new targets for immune based interventions. |
HostingRepository | PRIDE |
AnnounceDate | 2017-02-28 |
AnnouncementXML | Submission_2017-02-28_06:02:06.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ben Collins |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-10-27 23:58:58 | ID requested | |
⏵ 1 | 2017-02-28 06:02:07 | announced | |
Publication List
Nemeth J, Vongrad V, Metzner KJ, Strouvelle VP, Weber R, Pedrioli P, Aebersold R, G, ΓΌ, nthard HF, Collins BC, T Cells. Mol Cell Proteomics, 16(4 suppl 1):S108-S123(2017) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: HIV, SWATH, CD4 T cell |
Contact List
Ben Collins |
contact affiliation | Department of Biology Institute of Molecular Systems Biology ETH Zurich Switzerland |
contact email | collins@imsb.biol.ethz.ch |
lab head | |
Ben Collins |
contact affiliation | ETH Zurich |
contact email | collins@imsb.biol.ethz.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD005234 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005234
- Label: PRIDE project
- Name: In vivo and in vitro proteome analysis of HIV-1 infected, human CD4+ T cells