PXD005233

PXD005233 is an original dataset announced via ProteomeXchange.

Title	MudPIT analysis of the proteins interacting with truncations of the mixed lineage leukemia (MLL/KMT2A)
Description	Plasmids encoding Halo-tagged mixed lineage leukemia (MLL)/ histone-lysine N-methyltransferase (KMT2A) proteins were transiently transfected into HEK293 cells. Four truncations of MLL were also expressed into HEK293 cells: MLL-CT, which only contains the C-terminus of MLL (2760-3972aa); MLL-Inter, which is lost in MLL chimeras due to chromosome translocation (T1: 1163-2158aa); a truncation of the N-terminus to the second PHD finger (T2: 1478-2158aa); and another truncation to the third PHD domain (T3: 1545-2158aa). Two days later, HEK293 cells were harvested and lysed with mammalian lysis buffer (Promega). Halo-tagged proteins were purified with HaloTag resin in the presence of Benzonase (Sigma) and eluted with TEV protease. The eluates were precipitated with TCA. After washing with acetone, the protein mixtures were digested with endoproteinase Lys-C and trypsin (Roche) and analyzed by MudPIT. Vector only (Halo Vector) transfected cells were used as negative controls. The Halo-tagged MLL internal region (MLL-Inter) was also purified from HEK293 cells treated with DMSO or interleukin 1 receptor associated kinase (IRAK1) inhibitor for 24 h. Two biological replicate analyses were performed.
HostingRepository	MassIVE
AnnounceDate	2017-01-09
AnnouncementXML	Submission_2017-01-09_08:54:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laurence Florens
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-27 08:07:33	ID requested
⏵ 1	2017-01-09 08:54:53	announced

Liang K, Volk AG, Haug JS, Marshal SA, Woodfin AR, Bartom ET, Gilmore JM, Florens L, Washburn MP, Sullivan KD, Espinosa JM, Cannova J, Zhang J, Smith ER, Crispino JD, Shilatifard A. Therapeutic Targeting of MLL Degradation Pathways in MLL-Rearranged Leukemia. Cell 2017 Jan 12;168(1):1�14. doi: 10.1016/j.cell.2016.12.011.

submitter keyword: mixed lineage leukemia, histone-lysine N-methyltransferase, interleukin 1 receptor associated kinase, ubiquitin-conjugating enzyme E2 O, Complex of Proteins Associated with Set1 (COMPASS)

principal_investigator
lab head
Laurence Florens
contact affiliation	Stowers Institute for Medical Research
contact email	laf@stowers.org
dataset submitter

MassIVE dataset URI

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