PXD005229

PXD005229 is an original dataset announced via ProteomeXchange.

Title	The use of urinary proteomics in the assessment of suitability of mouse models for ageing.
Description	Ageing is a complex process characterised by a systemic and progressive deterioration of biological functions. As ageing is associated with an increased prevalence of age-related chronic disorders, understanding its underlying molecular mechanisms can pave the way for therapeutic interventions and managing complications. Animal models such as mice are commonly used in ageing research as they have a shorter lifespan in comparison to humans and are also genetically close to humans. To assess the translatability of mouse ageing to human ageing, the urinary proteome in 89 wild-type (C57BL/6) mice aged between 8-96 weeks was investigated using capillary electrophoresis coupled to mass spectrometry (CE-MS). Using age as a continuous variable, 295 peptides significantly correlated with age in mice were identified. To investigate the relevance of using mouse models in human ageing studies, a comparison was performed with a previous correlation analysis using 1227 healthy subjects. In mice and humans, a decrease in urinary excretion of fibrillar collagens and an increase of uromodulin fragments was observed with advanced age. Of the 295 peptides correlating with age, 49 had a strong homology to the respective human age-related peptides. These ortholog peptides including several collagen (N= 44) and uromodulin (N= 5) fragments were used to generate an ageing classifier that was able to discriminate the age among both wild-type mice and healthy subjects. Additionally, the ageing classifier depicted that telomerase knock-out mice were older than their chronological age. Hence, with a focus on ortholog urinary peptides mouse ageing can be translated to human ageing.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:33:37.228.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Esther Nkuipou Kenfack
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-27 00:02:25	ID requested
1	2017-02-21 06:54:23	announced
⏵ 2	2024-10-22 04:33:39	announced	2024-10-22: Updated project metadata.

Nkuipou-Kenfack E, Schanstra JP, Bajwa S, Pejchinovski M, Vinel C, Dray C, Valet P, Bascands JL, Vlahou A, Koeck T, Borries M, Busch H, Bechtel-Walz W, Huber TB, Rudolph KL, Pich A, Mischak H, Z, ü, rbig P, The use of urinary proteomics in the assessment of suitability of mouse models for ageing. PLoS One, 12(2):e0166875(2017) [pubmed]

10.1371/journal.pone.0166875;

submitter keyword: urine

proteome analysis

ageing

animal models

Petra Zürbig
contact affiliation	mosaiques diagnostics GmbH Rotenburger Str. 20 30659 Hannover Germany
contact email	zuerbig@mosaiques-diagnostics.com
lab head
Esther Nkuipou Kenfack
contact affiliation	Mosaiques diagnostics GmbH
contact email	nkuipou-kenfack@mosaiques.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD005229

PRIDE project URI

• PRIDE
  1. PXD005229
     1. Label: PRIDE project
     2. Name: The use of urinary proteomics in the assessment of suitability of mouse models for ageing.