PXD005215

PXD005215 is an original dataset announced via ProteomeXchange.

Title	iTRAQ Analysis of DJ-1 Knockout Rodent Brains
Description	Mutations in the DJ-1 (Park7) gene cause autosomal recessive Parkinson's disease in humans, but the function of the DJ-1 protein is poorly characterized. In an effort to understand more about the biology of DJ-1, we performed iTRAQ analyses on subcellular fractions enriched from DJ-1 knockout rat and mouse brains. We generated iTRAQ datasets for mitochondria and cytosol enriched fractions from 6-month-old rat brains, and the cytosol enriched fraction from 14-15-month old mouse brains. Our subsequent analyses of these datasets led to our discovery that the Hexokinase 1 protein was increased in the cytosol components of both DJ-1 knockout species.
HostingRepository	PRIDE
AnnounceDate	2017-10-04
AnnouncementXML	Submission_2017-10-04_02:53:05.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005215
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jinhui Ding
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	Methylthio; Oxidation; iTRAQ8plex
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-24 04:27:59	ID requested
⏵ 1	2017-10-04 02:53:06	announced

Hauser DN, Mamais A, Conti MM, Primiani CT, Kumaran R, Dillman AA, Langston RG, Beilina A, Garcia JH, Diaz-Ruiz A, Bernier M, Fiesel FC, Hou X, Springer W, Li Y, de Cabo R, Cookson MR, Hexokinases link DJ-1 to the PINK1/parkin pathway. Mol Neurodegener, 12(1):70(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: iTRAQ, DJ-1, neurodegeneration, Parkinson's, subcellular fractionation, mitochondria, cytosol, hexokinase, aging

Mark R Cookson
contact affiliation	Laboratory of Neurogenetics National Institute on Aging National Institutes of Health Building 35, Room 1A116 35 Convent Drive Bethesda, MD 20814, USA
contact email	cookson@mail.nih.gov
lab head
Jinhui Ding
contact affiliation	NIH
contact email	dingj@mail.nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD005215

PRIDE project URI

• PRIDE
  1. PXD005215
     1. Label: PRIDE project
     2. Name: iTRAQ Analysis of DJ-1 Knockout Rodent Brains