PXD005207

PXD005207 is an original dataset announced via ProteomeXchange.

Title	PfCDPK1 mediated signaling in erythrocytic stages of Plasmodium falciparum
Description	Calcium Dependent Protein Kinases (CDPKs) are key effectors of calcium signaling in malaria parasite. We have elucidated a novel role of PfCDPK1, which is indispensable for the erythrocytic development of Plasmodium falciparum, in invasion of host Red Blood Cells (RBCs). To gain insights into the mechanisms via which PfCDPK1 is involved in parasitic processes, a comparative phosphoproteomic analysis was employed to identify its parasitic targets. Proteins were isolated from CDPK1 knockdown and wild type parasites and processed for trypsin digestion and iTRAQ labeling. Quantitative proteomic analysis using high resolution mass spectrometry led to the identification of hypophosphorylated proteins, which can be the likely substrates for this kinase. In addition, in vitro kinase assays and antibody based validation of the hypophosphorylated sites was carried out. This study will provide a novel insight into the CDPK1 mediated invasion by Plasmodium falciparum.
HostingRepository	PRIDE
AnnounceDate	2017-05-02
AnnouncementXML	Submission_2017-09-13_00:09:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Keshava Prasad T. S.
SpeciesList	scientific name: Plasmodium falciparum; NCBI TaxID: 5833;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-24 02:01:17	ID requested
1	2017-05-02 05:15:46	announced
⏵ 2	2017-09-13 00:09:26	announced	Updated publication reference for PubMed record(s): 28680058.

Kumar S, Kumar M, Ekka R, Dvorin JD, Paul AS, Madugundu AK, Gilberger T, Gowda H, Duraisingh MT, Keshava Prasad TS, Sharma P, PfCDPK1 mediated signaling in erythrocytic stages of Plasmodium falciparum. Nat Commun, 8(1):63(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Orbitrap Fusion, Phosphoproteome

Keshava Prasad
contact affiliation	Faculty Scientist, Institute of Bioinformatics, Bangalore, India
contact email	keshav@ibioinformatics.org
lab head
Keshava Prasad T. S.
contact affiliation	Institute of Bioinformatics, Bangalore, Karnataka, India.
contact email	keshav@ibioinformatics.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD005207

PRIDE project URI

• PRIDE
  1. PXD005207
     1. Label: PRIDE project
     2. Name: PfCDPK1 mediated signaling in erythrocytic stages of Plasmodium falciparum