PXD005202 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A SUMO-Dependent Protein Network Regulates Chromosome Congression During Oocyte Meiosis |
Description | During Caenorhabditis elegans oocyte meiosis, a multi-protein complex localised between homologous chromosomes, the ring complex (RC), promotes chromosome congression through the action of the chromokinesin KLP-19. While some RC components are known, the mechanism of RC assembly has remained obscure. A germline-specific screen identified KLP-19 as a SUMO substrate in vivo and we found that the SUMO E3 ligase GEI-17/PIAS is required for KLP-19 recruitment to the RC. Additionally, KLP-19 is efficiently sumoylated in vitro in a GEI-17-dependent manner. Further biochemical analysis showed that KLP-19 and GEI-17 are efficiently modified by SUMO and that GEI-17 and another RC component, the kinase BUB-1, can interact non-covalently with SUMO. While SUMO conjugation is required for RC assembly, we also provide evidence consistent with non-covalent SUMO interactions contributing to RC assembly in vivo. Our results highlight the importance of sumoylation and non-covalent SUMO interaction in regulating dynamic protein complex assembly during oocyte meiosis. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:13:34.216.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Triin Tammsalu |
SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-10-21 02:14:53 | ID requested | |
1 | 2020-10-15 05:50:22 | announced | |
⏵ 2 | 2024-10-22 05:13:35 | announced | 2024-10-22: Updated project metadata. |
Publication List
Pelisch F, Tammsalu T, Wang B, Jaffray EG, Gartner A, Hay RT, A SUMO-Dependent Protein Network Regulates Chromosome Congression during Oocyte Meiosis. Mol Cell, 65(1):66-77(2017) [pubmed] |
10.1016/j.molcel.2016.11.001; |
Keyword List
curator keyword: Biological |
submitter keyword: KLP-19, PIAS, C. elegans,Sumoylation sites |
Contact List
Ronald T. Hay |
contact affiliation | University of Dundee, UK |
contact email | r.t.hay@dundee.ac.uk |
lab head | |
Triin Tammsalu |
contact affiliation | University of Dundee |
contact email | t.tammsalu@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005202
- Label: PRIDE project
- Name: A SUMO-Dependent Protein Network Regulates Chromosome Congression During Oocyte Meiosis