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PXD005202

PXD005202 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA SUMO-Dependent Protein Network Regulates Chromosome Congression During Oocyte Meiosis
DescriptionDuring Caenorhabditis elegans oocyte meiosis, a multi-protein complex localised between homologous chromosomes, the ring complex (RC), promotes chromosome congression through the action of the chromokinesin KLP-19. While some RC components are known, the mechanism of RC assembly has remained obscure. A germline-specific screen identified KLP-19 as a SUMO substrate in vivo and we found that the SUMO E3 ligase GEI-17/PIAS is required for KLP-19 recruitment to the RC. Additionally, KLP-19 is efficiently sumoylated in vitro in a GEI-17-dependent manner. Further biochemical analysis showed that KLP-19 and GEI-17 are efficiently modified by SUMO and that GEI-17 and another RC component, the kinase BUB-1, can interact non-covalently with SUMO. While SUMO conjugation is required for RC assembly, we also provide evidence consistent with non-covalent SUMO interactions contributing to RC assembly in vivo. Our results highlight the importance of sumoylation and non-covalent SUMO interaction in regulating dynamic protein complex assembly during oocyte meiosis.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:13:34.216.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTriin Tammsalu
SpeciesList scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-10-21 02:14:53ID requested
12020-10-15 05:50:22announced
22024-10-22 05:13:35announced2024-10-22: Updated project metadata.
Publication List
Pelisch F, Tammsalu T, Wang B, Jaffray EG, Gartner A, Hay RT, A SUMO-Dependent Protein Network Regulates Chromosome Congression during Oocyte Meiosis. Mol Cell, 65(1):66-77(2017) [pubmed]
10.1016/j.molcel.2016.11.001;
Keyword List
curator keyword: Biological
submitter keyword: KLP-19, PIAS, C. elegans,Sumoylation sites
Contact List
Ronald T. Hay
contact affiliationUniversity of Dundee, UK
contact emailr.t.hay@dundee.ac.uk
lab head
Triin Tammsalu
contact affiliationUniversity of Dundee
contact emailt.tammsalu@dundee.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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