PXD005202

PXD005202 is an original dataset announced via ProteomeXchange.

Title	A SUMO-Dependent Protein Network Regulates Chromosome Congression During Oocyte Meiosis
Description	During Caenorhabditis elegans oocyte meiosis, a multi-protein complex localised between homologous chromosomes, the ring complex (RC), promotes chromosome congression through the action of the chromokinesin KLP-19. While some RC components are known, the mechanism of RC assembly has remained obscure. A germline-specific screen identified KLP-19 as a SUMO substrate in vivo and we found that the SUMO E3 ligase GEI-17/PIAS is required for KLP-19 recruitment to the RC. Additionally, KLP-19 is efficiently sumoylated in vitro in a GEI-17-dependent manner. Further biochemical analysis showed that KLP-19 and GEI-17 are efficiently modified by SUMO and that GEI-17 and another RC component, the kinase BUB-1, can interact non-covalently with SUMO. While SUMO conjugation is required for RC assembly, we also provide evidence consistent with non-covalent SUMO interactions contributing to RC assembly in vivo. Our results highlight the importance of sumoylation and non-covalent SUMO interaction in regulating dynamic protein complex assembly during oocyte meiosis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:13:34.216.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Triin Tammsalu
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-21 02:14:53	ID requested
1	2020-10-15 05:50:22	announced
⏵ 2	2024-10-22 05:13:35	announced	2024-10-22: Updated project metadata.

Pelisch F, Tammsalu T, Wang B, Jaffray EG, Gartner A, Hay RT, A SUMO-Dependent Protein Network Regulates Chromosome Congression during Oocyte Meiosis. Mol Cell, 65(1):66-77(2017) [pubmed]

10.1016/j.molcel.2016.11.001;

curator keyword: Biological

submitter keyword: KLP-19, PIAS, C. elegans,Sumoylation sites

Ronald T. Hay
contact affiliation	University of Dundee, UK
contact email	r.t.hay@dundee.ac.uk
lab head
Triin Tammsalu
contact affiliation	University of Dundee
contact email	t.tammsalu@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005202
     1. Label: PRIDE project
     2. Name: A SUMO-Dependent Protein Network Regulates Chromosome Congression During Oocyte Meiosis