PXD005199
PXD005199 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteomics of FGF signaling in Chrondrocytes |
Description | To understand the determinants of chondrocyte FGF inhibitory response we sought to identify the unique set of proteins whose phosphorylation status is changed upon FGF treatment. RCS (Rat ChondroSarcoma) (RCS) cells were used for our experiments as a well-accepted model of proliferative chondrocytes which respond to FGF signaling with growth arrest Chondrocytes were treated with FGF1 and, as a control, cell cycle changes were monitored by FACScan analysis to demonstrate strong (around 95%) downregulation of S-phase at 24h. Three independent experiments were performed to obtain statistically representative datasets. We quantified 8299 proteins were quantified in the global proteome analysis using only unique or and razor peptides for quantitation . All proteins were identified in at least two replicates of a single treatment set by 2 or more peptides with 1% FDR Inclusion requirements for a single proteins were 1) two or more peptides identified with 51% FDR and 2) peptides that proteins were identified in at least 2 replicates of a single sample set. |
HostingRepository | MassIVE |
AnnounceDate | 2016-10-21 |
AnnouncementXML | Submission_2016-10-21_02:55:09.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jessica Chapman |
SpeciesList | scientific name: Rattus norvegicus; common name: Norway rat; NCBI TaxID: 10116; |
ModificationList | Thiophospho |
Instrument | Orbitrap Fusion ETD; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2016-10-20 19:43:16 | ID requested | |
⏵ 1 | 2016-10-21 02:55:09 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: FGF, phosphorylation, chrondocytes |
Contact List
Beatrix Ueberheide | |
---|---|
lab head | |
Jessica Chapman | |
contact affiliation | NYU School of Medicine |
contact email | jessica.chapman@nyumc.org |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000080259 |