PXD005198

PXD005198 is an original dataset announced via ProteomeXchange.

Title	GWI Mouse model SIDL - Complementary proteomic approaches reveal mitochondrial dysfunction, immune and inflammatory dysregulation in a mouse model of gulf war illness
Description	Long term consequences of combined pyridostigmine bromide and permethrin exposure in C57BL6/J mice using a well characterized mouse model of exposure to these Gulf War agents. Expanding on earlier work, we used orthogonal proteomic approaches to identify pathways that are chronically impacted in the mouse CNS due to semi-acute GW agent exposure early in life. These analyses were performed on soluble and membrane-bound protein fractions from brain samples using two orthogonal isotopic labeling LC-MS/MS proteomic approaches – stable isotope dimethyl labeling (SIDL) and isobaric tags for relative and absolute quantitation (iTRAQ). The use of these approaches allowed for greater coverage of proteins than was possible by either one alone and revealed both distinct and overlapping datasets. This combined analysis identified changes in several mitochondrial, as well as immune and inflammatory pathways after GW agent exposure. The work discussed here provides insight into GW-agent exposure dependent mechanisms that adversely affect mitochondrial function and immune and inflammatory regulation at five months post exposure to PB+PER.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:33:32.003.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gogce Crynen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	2x(13)C,6x(2)H labeled dimethylated L-arginine; dimethylated L-arginine; methylated residue; deaminated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-20 08:42:24	ID requested
1	2017-02-01 10:26:13	announced
2	2017-05-12 01:41:44	announced	Updated publication reference for PubMed record(s): 28371386.
⏵ 3	2024-10-22 04:33:34	announced	2024-10-22: Updated project metadata.

Zakirova Z, Reed J, Crynen G, Horne L, Hassan S, Mathura V, Mullan M, Crawford F, Ait-Ghezala G, Complementary proteomic approaches reveal mitochondrial dysfunction, immune and inflammatory dysregulation in a mouse model of Gulf War Illness. Proteomics Clin Appl, 11(9-10):(2017) [pubmed]

10.1002/prca.201600190;

curator keyword: Biomedical

submitter keyword: SIDL,GWI,Mouse model, gulf war illness

Ghania Ait Ghezala
contact affiliation	RoskampInstitute
contact email	gaitghezala@rfdn.org
lab head
Gogce Crynen
contact affiliation	Roskamp Institute
contact email	gkayihan@rfdn.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD005198

PRIDE project URI

• PRIDE
  1. PXD005198
     1. Label: PRIDE project
     2. Name: GWI Mouse model SIDL - Complementary proteomic approaches reveal mitochondrial dysfunction, immune and inflammatory dysregulation in a mouse model of gulf war illness