PXD005196

PXD005196 is an original dataset announced via ProteomeXchange.

Title	Characterisation of urinary WFDC12 in nocturnal basal primates, mouse lemurs (Microcebus spp.), C4PR_LIV
Description	Mouse lemurs are basal primates that rely on chemo- and acoustic signalling for social interactions in their dispersed social systems. We examined the urinary protein content of two mouse lemurs species, within and outside the breeding season, to assess candidates used in species discrimination, reproductive or competitive communication. Urine from Microcebus murinus and Microcebus lehilahytsara contain a predominant 10kDa protein, expressed in both species by some, but not all, males during the breeding season, but at very low levels by females. Mass spectrometry of the intact proteins confirmed the protein mass and revealed a 30 Da mass difference between proteins from the two species. Tandem mass spectrometry after digestion with three proteases and sequencing de novo defined the complete protein sequence and located an Ala/Thr difference between the two species that explained the 30 Da mass difference. The protein (mature form: 87 amino acids) is an atypical member of the whey acidic protein family (WFDC12). Seasonal excretion of this protein, species difference and male-specific expression during the breeding season suggest that it may have a function in intra- and/or intersexual chemical signalling in the context of reproduction, and could be a cue for sexual selection and species recognition.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_05:42:51.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005196
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Grace Loxley
SpeciesList	scientific name: Microcebus murinus; NCBI TaxID: 30608;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-20 03:28:06	ID requested
1	2017-02-13 03:27:52	announced
2	2017-02-28 09:41:44	announced	Updated publication reference for PubMed record(s): 28225021.
3	2017-03-02 00:30:20	announced	Updated project metadata.
⏵ 4	2017-10-24 05:42:52	announced	Updated project metadata.

Unsworth J, Loxley GM, Davidson A, Hurst JL, G, ó, mez-Baena G, Mundy NI, Beynon RJ, Zimmermann E, Radespiel U, Characterisation of urinary WFDC12 in small nocturnal basal primates, mouse lemurs (Microcebus spp.). Sci Rep, 7():42940(2017) [pubmed]

curator keyword: Biological

submitter keyword: WFDC12 protein, Whey acidic protein, mass spectrometry, urine, Microcebus murinus, Microcebus lehilahytsara, reproduction

Robert J Beynon
contact affiliation	Centre for Proteome Research, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK
contact email	rbeynon@liverpool.ac.uk
lab head
Grace Loxley
contact affiliation	University of Liverpool
contact email	G.M.Loxley@liverpool.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005196
     1. Label: PRIDE project
     2. Name: Characterisation of urinary WFDC12 in nocturnal basal primates, mouse lemurs (Microcebus spp.), C4PR_LIV