PXD005185 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Gulf War Illness mouse model chronic iTRAQ |
Description | Long term consequences of combined pyridostigmine bromide and permethrin exposure in C57BL6/J mice using a well characterized mouse model of exposure to these Gulf War agents. Expanding on earlier work, we used orthogonal proteomic approaches to identify pathways that are chronically impacted in the mouse CNS due to semi-acute GW agent exposure early in life. These analyses were performed on soluble and membrane-bound protein fractions from brain samples using two orthogonal isotopic labeling LC-MS/MS proteomic approaches – stable isotope dimethyl labeling (SIDL) and isobaric tags for relative and absolute quantitation (iTRAQ). The use of these approaches allowed for greater coverage of proteins than was possible by either one alone and revealed both distinct and overlapping datasets. This combined analysis identified changes in several mitochondrial, as well as immune and inflammatory pathways after GW agent exposure. The work discussed here provides insight into GW-agent exposure dependent mechanisms that adversely affect mitochondrial function and immune and inflammatory regulation at five months post exposure to PB+PER |
HostingRepository | PRIDE |
AnnounceDate | 2017-02-01 |
AnnouncementXML | Submission_2017-05-12_01:42:21.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gogce Crynen |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iTRAQ4plex-116 reporter+balance reagent acylated residue; methylated residue; monohydroxylated residue; symmetric dimethyl-L-arginine; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-10-19 01:42:02 | ID requested | |
1 | 2017-02-01 10:46:02 | announced | |
⏵ 2 | 2017-05-12 01:42:23 | announced | Updated publication reference for PubMed record(s): 28371386. |
Publication List
Zakirova Z, Reed J, Crynen G, Horne L, Hassan S, Mathura V, Mullan M, Crawford F, Ait-Ghezala G, Complementary proteomic approaches reveal mitochondrial dysfunction, immune and inflammatory dysregulation in a mouse model of Gulf War Illness. Proteomics Clin Appl, 11(9-10):(2017) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Gulf War Illness, mouse model, chronic, iTRAQ, quantitative proteomics |
Contact List
Ghania Ait-Ghezala |
contact affiliation | Roskamp Institute |
contact email | GAitghezala@RFDN.ORG |
lab head | |
Gogce Crynen |
contact affiliation | Roskamp Institute |
contact email | gkayihan@rfdn.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005185
- Label: PRIDE project
- Name: Gulf War Illness mouse model chronic iTRAQ