PXD005184

PXD005184 is an original dataset announced via ProteomeXchange.

Title	Discovery of Age-Related Protein Folding Stability Differences in the Mouse Brain Proteome
Description	Described here is the application of thermodynamic stability measurements to study age-related differences in the protein folding and stability of proteins in a rodent model of ageing. Using the Stability of Proteins from Rates of Oxidation (SPROX) technique the thermodynamic stability profiles were generated for 807 proteins in brain cell lystaes from mice, aged 6- (n=7) and 18-months (n=9). The biological variability of the protein stability measurements was low and within the experimental error of SPROX. A total of 83 protein hits were detected with age-related stability differences in the brain samples. Remarkably, the large majority of the brain protein hits were destabilized in the old mice, and the hits were enriched in proteins that have slow turnover rates (p <0.07). Furthermore, 70% of the hits have been previously linked to ageing or age-related diseases. These results help validate the use of thermodynamic stability measurements to capture relevant age-related proteomic changes, and they establish a new biophysical link between these proteins and ageing.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:33:31.839.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julia Roberts
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	methylthiolated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-19 01:30:10	ID requested
1	2016-12-22 08:25:56	announced
2	2017-10-24 03:20:37	announced	Updated project metadata.
⏵ 3	2024-10-22 04:33:39	announced	2024-10-22: Updated project metadata.

Roberts JH, Liu F, Karnuta JM, Fitzgerald MC, Discovery of Age-Related Protein Folding Stability Differences in the Mouse Brain Proteome. J Proteome Res, 15(12):4731-4741(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Mouse, ageing, SPROX, iTRAQ

Michael C. Fitzgerald
contact affiliation	Department of Chemistry, Duke University
contact email	michael.c.fitzgerald@duke.edu
lab head
Julia Roberts
contact affiliation	Duke University
contact email	jhr21@duke.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD005184

PRIDE project URI

• PRIDE
  1. PXD005184
     1. Label: PRIDE project
     2. Name: Discovery of Age-Related Protein Folding Stability Differences in the Mouse Brain Proteome