⮝ Full datasets listing

PXD005184

PXD005184 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDiscovery of Age-Related Protein Folding Stability Differences in the Mouse Brain Proteome
DescriptionDescribed here is the application of thermodynamic stability measurements to study age-related differences in the protein folding and stability of proteins in a rodent model of ageing. Using the Stability of Proteins from Rates of Oxidation (SPROX) technique the thermodynamic stability profiles were generated for 807 proteins in brain cell lystaes from mice, aged 6- (n=7) and 18-months (n=9). The biological variability of the protein stability measurements was low and within the experimental error of SPROX. A total of 83 protein hits were detected with age-related stability differences in the brain samples. Remarkably, the large majority of the brain protein hits were destabilized in the old mice, and the hits were enriched in proteins that have slow turnover rates (p <0.07). Furthermore, 70% of the hits have been previously linked to ageing or age-related diseases. These results help validate the use of thermodynamic stability measurements to capture relevant age-related proteomic changes, and they establish a new biophysical link between these proteins and ageing.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:33:31.839.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJulia Roberts
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmethylthiolated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-10-19 01:30:10ID requested
12016-12-22 08:25:56announced
22017-10-24 03:20:37announcedUpdated project metadata.
32024-10-22 04:33:39announced2024-10-22: Updated project metadata.
Publication List
Roberts JH, Liu F, Karnuta JM, Fitzgerald MC, Discovery of Age-Related Protein Folding Stability Differences in the Mouse Brain Proteome. J Proteome Res, 15(12):4731-4741(2016) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: Mouse, ageing, SPROX, iTRAQ
Contact List
Michael C. Fitzgerald
contact affiliationDepartment of Chemistry, Duke University
contact emailmichael.c.fitzgerald@duke.edu
lab head
Julia Roberts
contact affiliationDuke University
contact emailjhr21@duke.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD005184
PRIDE project URI
Repository Record List
[ + ]