PXD005174

PXD005174 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Phosphoproteomic data of HCT116 cells
Description	Quantitative phosphoproteome analyses of colorectal cancer cell lines (HCT116 metastatic clone and the parent clone without metastasis) were carried out using IMAC-based posphopeptide enrichment followed by SCX-RP-MS/MS with iTRAQ labeling(metastasis:iTRAQ116 or 117, no metastasis: iTRAQ114 or 115). The obtained results were searched against UniProt DB by Mascot, and the identified peptides were filtered at 1% FDR thresholds by searching against the randomized decoy DB at peptide level. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project.
HostingRepository	jPOST
AnnounceDate	2016-10-18
AnnouncementXML	Submission_2022-09-18_03:11:30.944.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005174
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mariko Ito
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; iTRAQ4plex-116 reporter+balance reagent N6-acylated lysine; iTRAQ4plex-116 reporter+balance reagent acylated N-terminal; iTRAQ4plex-116 reporter+balance reagent O4'-acylated tyrosine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-17 08:15:21	ID requested
1	2016-10-17 21:28:39	announced
2	2017-03-30 23:37:44	announced	Updated Repository support.
3	2017-03-31 00:16:48	announced	Updated DigitalObjectIdentifier.
4	2018-09-18 23:45:11	announced	Updated CV, FTP location and Modification list.
5	2021-02-04 17:16:10	announced	2021-02-04: Updated FTP location.
⏵ 6	2022-09-18 03:11:31	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: colorectal cancer
FASP
IMAC
missing protein
phosphopeptide
PhosphoSitePlus

submitter keyword: colorectal cancer

FASP

IMAC

missing protein

phosphopeptide

PhosphoSitePlus

Contact List

Takeshi Tomonaga
lab head
Mariko Ito
contact affiliation	Kyoto Univ.
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000206/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000206/