PXD005168

PXD005168 is an original dataset announced via ProteomeXchange.

Title	Persulfidated subproteome reveals the regulation of protein function by hydrogen sulfide in diverse biological processes and nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase in plants.
Description	Hydrogen sulfide-mediated signaling pathways regulates many physiological andpathophysiological processes in mammalian and plant systems. The molecular mechanism bywhich hydrogen sulfide exerts its action involves the oxidative post-translational modificationof cysteine residues to form a persulfidated thiol motif, named as protein S-sulfhydration orpersulfidation. We have developed a comparative and quantitative proteomic analysisapproach for the detection of endogenous S-sulfhydrated proteins in wild-type Arabidopsisand L-CYSTEINE DESULFHYDRASE 1 mutant leaves by using the tag-switch method.Bioinformatic analysis of the identified proteins revealed that S-sulfhydrated cysteines arepart of a wide range of biological functions regulating important processes such as carbonmetabolism, plant responses to abiotic and biotic stresses, plant growth and development, andRNA translation. Quantitative analysis in both genetic backgrounds reveals hydrogen sulfidemediatedprotein sulfhydration as a new regulatory component in primary metabolic pathwayssuch as tricarboxylic acid cycle, glycolysis or Calvin cycle. In addition, studies on thesubcellular localization of glyceraldehyde-3-phosphate dehydrogenase show that sulfideregulates the cytosolic/nuclear partitioning by enhancing the nuclear localization of theprotein and appears to be a critical step for reprogramming cellular metabolism.
HostingRepository	PRIDE
AnnounceDate	2017-10-27
AnnouncementXML	Submission_2017-10-27_06:38:04.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005168
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sergio Ciordia Higuera
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	Sulfide; Methylthio; Oxidation; Acetyl; Oxidation
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-17 02:23:02	ID requested
⏵ 1	2017-10-27 06:38:05	announced

Aroca A, Benito JM, Gotor C, Romero LC, Persulfidation proteome reveals the regulation of protein function by hydrogen sulfide in diverse biological processes in Arabidopsis. J Exp Bot, 68(17):4915-4927(2017) [pubmed]

curator keyword: Biological

submitter keyword: S-sulfhydration, Hydrogen sulfide, Postranslational modification, Arabidopsis, GAPDH, nuclear localization

Luis C. Romero
contact affiliation	Instituto de Bioquímica Vegetal y Fotosíntesis (IBVF-CSIC)
contact email	lromero@ibvf.csic.es
lab head
Sergio Ciordia Higuera
contact affiliation	Proteomics Unit
contact email	proteomics.masas@cnb.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005168
     1. Label: PRIDE project
     2. Name: Persulfidated subproteome reveals the regulation of protein function by hydrogen sulfide in diverse biological processes and nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase in plants.