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PXD005163

PXD005163 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIn-depth membrane proteomic study of breast cancer tissues
DescriptionHuman breast cancer tissue samples from 18 patients with high-risk or low-risk MammaPrint breast cancer were analyzed. The membrane fractions were enriched, and the extracted proteins were reduced, alkylated, digested with Lys-C and Trypsin, and labelled with iTRAQ4plex: 114; high risk; 115; low risk. Identification results were filtered at 1% false discovery rate (FDR) thresholds by searching against a randomized decoy database using Mascot at peptide and protein levels.
HostingRepositoryjPOST
AnnounceDate2016-10-17
AnnouncementXMLSubmission_2022-09-18_03:21:49.480.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD005163
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMariko Ito
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; iTRAQ4plex-116 reporter+balance reagent N6-acylated lysine; iTRAQ4plex-116 reporter+balance reagent acylated N-terminal; iTRAQ4plex-116 reporter+balance reagent O4'-acylated tyrosine
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-10-17 01:22:12ID requested
12016-10-17 04:05:10announced
22016-11-17 18:13:15announcedUpdated description.
32017-03-30 23:34:44announcedUpdated Repository support.
42017-03-31 00:12:18announcedUpdated DigitalObjectIdentifier.
52018-09-18 23:41:05announcedUpdated CV, FTP location and Modification list.
62021-02-04 17:13:27announced2021-02-04: Updated FTP location.
72022-09-18 03:21:49announced2022-09-18: Updated FTP location.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: membrane protein
breast cancer tissues
shotgun proteomics
Contact List
Takeshi Tomonaga
lab head
Mariko Ito
contact affiliationKyoto Univ.
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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