PXD005160

PXD005160 is an original dataset announced via ProteomeXchange.

Title	Seminal fluid proteome of the polyandrous Red junglefowl
Description	We characterized the SF proteome of the polyandrous Red junglefowl, Gallus gallus, the wild species that gave rise to the domestic chicken. We identify 1,141 SFPs, including proteins involved in immunity and antimicrobial defences, sperm maturation, and fertilisation, revealing a functionally complex SF proteome. This includes a predominant contribution of blood plasma proteins that is conserved with human SF. By comparing the proteome of young and old males with fast or slow sperm velocity in a balanced design, we identify proteins associated with ageing and sperm velocity, and show that old males that retain high sperm velocity have distinct proteome characteristics. SFP comparisons with domestic chickens revealed both qualitative and quantitative differences likely associated with domestication and artificial selection. Collectively, these results shed light onto the functional complexity of avian SF, and provide a platform for molecular studies of fertility, reproductive ageing, and domestication.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_06:05:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emma Whittington
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-17 01:10:22	ID requested
1	2017-01-03 03:34:46	announced
2	2017-10-24 04:00:16	announced	Updated project metadata.
⏵ 3	2017-10-24 06:05:16	announced	Updated project metadata.

Borziak K, Á, lvarez-Fern, á, ndez A, L Karr T, Pizzari T, Dorus S, The Seminal fluid proteome of the polyandrous Red junglefowl offers insights into the molecular basis of fertility, reproductive ageing and domestication. Sci Rep, 6():35864(2016) [pubmed]

curator keyword: Biological

submitter keyword: red junglefowl, seminal fluid, proteomics, fertility, reproductive ageing, domestication

Dr Steve Dorus
contact affiliation	Syracuse University
contact email	sdorus@syr.edu
lab head
Emma Whittington
contact affiliation	Syracuse Univeristy
contact email	ewhittin@syr.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD005160

PRIDE project URI

• PRIDE
  1. PXD005160
     1. Label: PRIDE project
     2. Name: Seminal fluid proteome of the polyandrous Red junglefowl