PXD005145

PXD005145 is an original dataset announced via ProteomeXchange.

Title	Comparison of Intact Glycopeptide Enrichment Methods Using Strong Anion Exchange and Hydrophilic Interaction Liquid Chromatography
Description	Heterogeneity of protein glycosylation poses great challenge for analysis that is key to un-puzzle systems glycobiology in diseases. Resolving this conundrum requires global enrichment of glycopeptides for identification and quantitation. To this aim, hydrophilic interaction chromatography (HILIC) has been proved to be an effective approach to enrich N-linked glycopeptides (N-glycopeptides). However, its effectiveness to enrich O-linked glycopeptides (O-glycopeptides) and isobaric labelled glycopeptides remains unclear. Here, we studied three different cartridges in HILIC mode. It was found that removal of N-glycosylation prior to enrichment improved identification of O-glycopeptides. It was noted that increased yield and number of glycosylation identification were seen when using cartridges having materials for strong anion exchange (SAX) chromatography. Remarkably, O-glycopeptides were found to be selectively enriched by HILIC with further improved enrichment being seen when Retain AX cartridge being used. Of particular note, isobaric labelled glycopeptides could be readily enriched by SAX cartridges in HILIC mode to enable quantitative glycoproteomics. It is anticipated that the use of SAX cartridges will facilitate broad applications of qualitative and quantitative glycoproteomics in diseases.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:33:20.324.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Weiming Yang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-13 02:29:55	ID requested
1	2017-10-19 00:01:45	announced
⏵ 2	2024-10-22 04:33:27	announced	2024-10-22: Updated project metadata.

10.1021/acs.analchem.7b03641;

Yang W, Shah P, Hu Y, Toghi Eshghi S, Sun S, Liu Y, Zhang H, Comparison of Enrichment Methods for Intact N- and O-Linked Glycopeptides Using Strong Anion Exchange and Hydrophilic Interaction Liquid Chromatography. Anal Chem, 89(21):11193-11197(2017) [pubmed]

curator keyword: Biological

submitter keyword: glycoproteomics, HILIC, SAX, quantitation, O-linked

Hui Zhang
contact affiliation	Department of Pathology, School of Medicine, Johns Hopkins University
contact email	huizhang@jhu.edu
lab head
Weiming Yang
contact affiliation	Johns Hopkins University
contact email	wesley.young@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005145
     1. Label: PRIDE project
     2. Name: Comparison of Intact Glycopeptide Enrichment Methods Using Strong Anion Exchange and Hydrophilic Interaction Liquid Chromatography