PXD005142

PXD005142 is an original dataset announced via ProteomeXchange.

Title	TORC1 shapes the SUMO proteome to control RNA Polymerase III activity
Description	Loss of nutrient supply elicits alterations of the SUMO proteome and sumoylation is crucial to various cellular processes including transcription. However, the physiological significance of sumoylation of transcriptional regulators is unclear. To begin clarifying this, we mapped the SUMO proteome under nitrogen-limiting conditions in Saccharomyces cerevisiae. Interestingly, several RNA polymerase III (RNAPIII) components are major SUMO targets under normal growth conditions, including Rpc53, Rpc82, and Ret1, and nutrient starvation results in rapid desumoylation of these proteins. These findings are supported by ChIP-seq experiments that show that SUMO is highly enriched at tDNA genes. Furthermore, RNA-seq experiments revealed that preventing sumoylation results in significantly decreased tRNA transcription. TORC1 inhibition resulted in the same effect, and our data indicate that the SUMO and TORC1 pathways are both required for robust tDNA expression. Importantly, tRNA transcription was strongly reduced in cells expressing a non-sumoylatable Rpc82-4KR mutant, which correlated with a misassembled RNAPIII transcriptional complex. Our data suggest that in addition to TORC1 activity, sumoylation of RNAPIII is key to reaching full translational capacity under optimal growth conditions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:34:26.457.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joseph Robertson
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-13 02:15:41	ID requested
1	2017-01-23 01:27:13	announced
⏵ 2	2024-10-22 04:34:29	announced	2024-10-22: Updated project metadata.

10.1073/pnas.1615093114;

Chymkowitch P, Ngu, é, a P A, Aanes H, Robertson J, Klungland A, Enserink JM, TORC1-dependent sumoylation of Rpc82 promotes RNA polymerase III assembly and activity. Proc Natl Acad Sci U S A, 114(5):1039-1044(2017) [pubmed]

curator keyword: Biological

submitter keyword: transcription, SUMOylation, RNA polymerase,Saccharomyces cerevisiae, ChIP-Seq, LC-MS/MS

Jorrit Enserink
contact affiliation	Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway
contact email	jorrit.enserink@rr-research.no
lab head
Joseph Robertson
contact affiliation	Oslo University Hospital
contact email	joseph.mark.robertson@rr-research.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005142
     1. Label: PRIDE project
     2. Name: TORC1 shapes the SUMO proteome to control RNA Polymerase III activity