PXD005133

PXD005133 is an original dataset announced via ProteomeXchange.

Title	Identification of proteins in a Salmonella SPI-1 type III secretion injectisome basal body mutant
Description	We determined the cryo-EM structure of a secretion incompetent PrgH130-392 type III secretion injectisome basal body mutant at 6.3 Å. The map shows no density attributable to the inner rod or needle and the periplasmic gate in a closed conformation. We used LC-MS/MS to idensitfy the constituent proteins showing the predominant presence of the major inner- and outer basal body proteins (PrgH+PrgK and InvG respectively) and export apparatus protein SpaP.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:32:40.464.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liam Worrall
SpeciesList	scientific name: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720); NCBI TaxID: 99287;
ModificationList	No PTMs are included in the dataset
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-12 01:12:30	ID requested
1	2017-01-02 12:54:42	announced
2	2017-10-24 03:09:10	announced	Updated project metadata.
⏵ 3	2024-10-22 04:32:43	announced	2024-10-22: Updated project metadata.

Worrall LJ, Hong C, Vuckovic M, Deng W, Bergeron JRC, Majewski DD, Huang RK, Spreter T, Finlay BB, Yu Z, Strynadka NCJ, Near-atomic-resolution cryo-EM analysis of the Salmonella T3S injectisome basal body. Nature, 540(7634):597-601(2016) [pubmed]

10.1038/nature20576;

submitter keyword: Salmonella, type iii secretion system, injectisome

Natalie Strynadka
contact affiliation	Department of Biochemistry, The University of British Columbia, Vancouver, Canada
contact email	ncjs@mail.ubc.ca
lab head
Liam Worrall
contact affiliation	The University of British Columbia
contact email	liam@byron.biochem.ubc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD005133

PRIDE project URI

• PRIDE
  1. PXD005133
     1. Label: PRIDE project
     2. Name: Identification of proteins in a Salmonella SPI-1 type III secretion injectisome basal body mutant