PXD005132

PXD005132 is an original dataset announced via ProteomeXchange.

Title	Electrospray ionization in concentrated acetonitrile vapor improves the performance of mass spectrometry for proteomic analyses
Description	Suppressing the background interferences and enhancing the analyte signals are long-term goals in high performance electrospray ionization mass spectrometry (ESI-MS) analyses. We observed that performing electrospray in the presence of a concentrated acetonitrile atmosphere suppresses background interferences and enhances peptide signals. An enclosed nanoESI source was utilized to provide a stable atmosphere of concentrated acetonitrile vapor for high performance ESI-MS analyses. The median MS signal intensity increased by 5 times for a set of 23 BSA tryptic peptides in direct nanoESI-MS analysis. Further, the number of reproducibly and precisely quantified peptides could be improved 67% in six replicate label-free quantitative proteome analyses by this strategy.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_06:13:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jin Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-11 07:27:57	ID requested
1	2017-01-04 06:04:22	announced
⏵ 2	2017-10-24 06:14:00	announced	Updated project metadata.

Chen J, Wang F, Liu Z, Liu J, Zhu Y, Zhang Y, Zou H, Electrospray ionization in concentrated acetonitrile vapor improves the performance of mass spectrometry for proteomic analyses. J Chromatogr A, 1483():101-109(2017) [pubmed]

curator keyword: Technical

submitter keyword: acetonitrile, background interference, mass spectrometry, electrospray ionization

Fangjun Wang
contact affiliation	Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, Liaoning, P. R. China.
contact email	wangfj@dicp.ac.cn
lab head
Jin Chen
contact affiliation	DICP
contact email	cjin350625@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005132
     1. Label: PRIDE project
     2. Name: Electrospray ionization in concentrated acetonitrile vapor improves the performance of mass spectrometry for proteomic analyses