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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2017-04-20
  • AnnouncementXML: Submission_2017-04-20_01:34:56.xml
  • DigitalObjectIdentifier: http://dx.doi.org/10.6019/PXD005130
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Xiaoke Yin
  • Title: Extracellular Matrix Proteomics Identifies Molecular Signature of Atherosclerotic Plaques from Symptomatic Patients
  • Description: Recent findings have challenged the prevailing histology- or imaging-based definition of the “vulnerable plaque”. To investigate molecular characteristics associated with “clinical instability” of atherosclerosis, we performed a proteomics comparison of the vascular extracellular matrix and associated molecules in human carotid endarterectomy specimens from symptomatic versus asymptomatic patients. The proteomics data were integrated with gene expression profiling and an analysis of protein secretion by lipid-loaded human vascular smooth muscle cells. The molecular signature of plaques from symptomatic patients identified by proteomics and at least one of the other two approaches comprised matrix metalloproteinase-9, chitinase-3-like protein 1, S100A8/S100A9, cathepsin B, fibronectin and galectin-3-binding protein. These biomarker candidates were measured in 685 subjects of the Bruneck Study and found to significantly predict the progression to advanced atherosclerosis (as assessed by repeated carotid ultrasound) and the incidence of cardiovascular disease over a 10-year follow-up period, highlighting the strength of tissue-based proteomics for biomarker discovery.
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: No PTMs are included in the dataset
  • Instrument: LTQ Orbitrap

Dataset History

VersionDatetimeStatusChangeLog Entry
02016-10-11 02:15:39ID requested
12017-04-20 01:34:57announced

Publication List

  1. Langley SR, Willeit K, Didangelos A, Matic LP, Skroblin P, Barallobre-Barreiro J, Lengquist M, Rungger G, Kapustin A, Kedenko L, Molenaar C, Lu R, Barwari T, Suna G, Yin X, Iglseder B, Paulweber B, Willeit P, Shalhoub J, Pasterkamp G, Davies AH, Monaco C, Hedin U, Shanahan CM, Willeit J, Kiechl S, Mayr M, Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques. J Clin Invest, 127(4):1546-1560(2017) [pubmed]

Keyword List

  1. curator keyword: Biomedical
  2. submitter keyword: atherosclerosis, carotid endarterectomy, gene expression, population study, biomarkers, proteomics

Contact List

    Manuel Mayr
    • contact affiliation: King's British Heart Foundation Centre, King's College London, London, United Kingdom
    • contact email: manuel.mayr@kcl.ac.uk
    • lab head:
    Xiaoke Yin
    • contact affiliation: Cardiovascular Division, King's College London
    • contact email: xiaoke.yin@kcl.ac.uk
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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