⮝ Full datasets listing

PXD005119

PXD005119 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleExtraordinary Diversity of Amyloid-beta Proteoforms in Human Alzheimer's Disease Brain
DescriptionAmyloid-beta (Aβ) plays a key role in the neuropathogenesis of Alzheimer’s disease, but little is known about the proteoforms (i.e., all protein variants of a single gene including post-translational modifications and sequence variants) present in human AD brain. We used high-resolution mass spectrometry to analyze intact, whole Aβ from soluble and insoluble aggregates in brains of 6 cases with severe dementia and pathologically confirmed Alzheimer’s disease. We found an extraordinary diversity of Aβ peptides totaling 91 unique proteoforms including various N- and C-terminal truncations, 17 different types of post-translational modifications (PTMs), and remarkably 5 amino acid variants in the peptide sequence never before described. Ratios of Aβ proteoforms did not distinguish between soluble and insoluble aggregates, but four individual Aβ proteoforms, three in the insoluble and one in the soluble fraction, were markedly increased when compared across aggregates. Such heterogeneity of the Aβ peptide both deepens our understanding of AD, but perhaps also warrants pause and consideration in our investigation into pathological mechanisms of the disease and therapeutic development.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:32:52.915.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNorelle Wildburger
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-10-10 01:44:32ID requested
12017-09-04 00:36:46announced
22024-10-22 04:32:53announced2024-10-22: Updated project metadata.
Publication List
Wildburger NC, Esparza TJ, LeDuc RD, Fellers RT, Thomas PM, Cairns NJ, Kelleher NL, Bateman RJ, Brody DL, Diversity of Amyloid-beta Proteoforms in the Alzheimer's Disease Brain. Sci Rep, 7(1):9520(2017) [pubmed]
10.1038/s41598-017-10422-x;
Keyword List
curator keyword: Biomedical
submitter keyword: Human AD Brain
nLC-MS/MS
Contact List
David Brody
contact affiliationProfessor of Neuology Washington University School of Medicine St. Louis, MO 61130
contact emailbrodyd@wustl.edu
lab head
Norelle Wildburger
contact affiliationWashington University
contact emailn.wildburger@wustl.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD005119
PRIDE project URI
Repository Record List
[ + ]