PXD005114

PXD005114 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Asymmetrically inherited older proteins in budding yeast (synchronized culture method-1)
Description	In budding yeast, damaged organelles and biomolecules are symmetrically segregated between mother and daughter cells, which can influence the replicating aging of mother cells. In some cases, older proteins are prone to be damaged and decline in their functions compared to newly synthesized proteins, likely to contribute the cellular aging. The purpose of this analysis is to uncover asymmetrically inherited older proteins, which are preferentially retained in mother cells during division into mother and rejuvenating daughter cells. To achieve this purpose, we conducted an originally developed strategy where mother and daughter cells are separated after just one cycle of synchronized culture, during which newly synthesized proteins are labeled with stable isotope amino acid. Synchronized culture was carried out using mating pheromone, which arrest the budding yeast in G1 phase. We successfully identified more than 20 proteins whose older forms are asymmetrically inherited by mother cells, among which are proteins involved in the intracellular homeostasis of the proton concentration and the response to the stress of misfolded proteins.
HostingRepository	jPOST
AnnounceDate	2017-10-08
AnnouncementXML	Submission_2022-09-18_02:33:29.469.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Keiji Kito
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; 6x(13)C labeled L-leucine
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-08 02:12:23	ID requested
1	2017-10-07 08:00:07	announced
2	2017-10-08 21:40:18	announced	Updated PubMed.
3	2018-09-18 23:36:56	announced	Updated CV, FTP location and Modification list.
4	2021-02-04 17:29:12	announced	2021-02-04: Updated FTP location.
⏵ 5	2022-09-18 02:33:30	announced	2022-09-18: Updated FTP location.

Publication List

Okada M, Kusunoki S, Ishibashi Y, Kito K, Proteomics analysis for asymmetric inheritance of preexisting proteins between mother and daughter cells in budding yeast. Genes Cells, 22(6):591-601(2017) [pubmed]

Okada M, Kusunoki S, Ishibashi Y, Kito K, Proteomics analysis for asymmetric inheritance of preexisting proteins between mother and daughter cells in budding yeast. Genes Cells, 22(6):591-601(2017) [pubmed]

Keyword List

submitter keyword: Asymmetric inheritance, Protein aging, Replicative aging, Budding yeast

submitter keyword: Asymmetric inheritance, Protein aging, Replicative aging, Budding yeast

Contact List

Keiji Kito
lab head
Keiji Kito
contact affiliation	Meiji University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000198/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000198/