PXD005107

PXD005107 is an original dataset announced via ProteomeXchange.

Title	AP-MS in CRISPR/Cas9 Utilizing Systems for Mapping Endogenous Protein Complexes
Description	We combined clustered regularly interspaced short palindromic repeats/Cas9-mediated gene knockout technology with affinity purification using antibodies against endogenous proteins followed by mass spectrometry analysis, to sensitively and precisely detect protein-protein interactions in unaltered in vivo settings. Using this system, we analyzed endogenous neurofibromin-associated protein complexes.
HostingRepository	PRIDE
AnnounceDate	2017-03-15
AnnouncementXML	Submission_2017-03-15_06:08:22.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005107
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Xu Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Label:13C(6); DeStreak; Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-06 00:38:03	ID requested
⏵ 1	2017-03-15 06:08:23	announced

Li X, Gao M, Choi JM, Kim BJ, Zhou MT, Chen Z, Jain AN, Jung SY, Yuan J, Wang W, Wang Y, Chen J, Clustered, Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9-coupled Affinity Purification/Mass Spectrometry Analysis Revealed a Novel Role of Neurofibromin in mTOR Signaling. Mol Cell Proteomics, 16(4):594-607(2017) [pubmed]

curator keyword: Technical, Biomedical

submitter keyword: ACUMEN, NF1, KRAS, AP-MS, CRISPR/Cas9

Junjie Chen
contact affiliation	Experimental Radiation Oncology, The University of Texas, MD Anderson Cancer Center
contact email	jchen8@mdanderson.org
lab head
Xu Li
contact affiliation	Experimental Radiation Oncology
contact email	xulimda@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/03/PXD005107

PRIDE project URI

• PRIDE
  1. PXD005107
     1. Label: PRIDE project
     2. Name: AP-MS in CRISPR/Cas9 Utilizing Systems for Mapping Endogenous Protein Complexes