PXD005106

PXD005106 is an original dataset announced via ProteomeXchange.

Title	Comparative Proteomics of PairedVocal Fold and Oral Mucosa Fibroblasts
Description	Injuries of the vocal folds frequently heal with scar formation, which can have lifelong detrimental impact on voice quality. Current treatments to prevent or resolve scars of the vocal fold mucosa are highly unsatisfactory. In contrast, the adjacent oral mucosa is mostly resistant to scarring. These differences in healing tendency might relate to distinct properties of the fibroblasts populating oral and vocal fold mucosae. We thus established the in vitro cultivation of paired, near-primary vocal fold fibroblasts (VFF) and oral mucosa fibroblasts (OMF) to perform a basic cellular characterization and comparative cellular proteomics. VFF were significantly larger than OMF, proliferated more slowly, and exhibited a sustained TGF-β1-induced elevation of pro-fibrotic interleukin 6. Cluster analysis of the proteomic data revealed distinct protein repertoires specific for VFF and OMF. Further, VFF displayed a broader protein spectrum, particularly a more sophisticated array of factors constituting and modifying the extracellular matrix. Conversely, subsets of OMF-enriched proteins were linked to cellular proliferation, nuclear events, and protection against oxidative stress. Altogether, this study supports the notion that fibroblasts sensitively adapt to the functional peculiarities of their respective anatomical location and presents several molecular targets for further investigation in the context of vocal fold wound healing.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:32:49.236.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barbara Darnhofer
SpeciesList	scientific name: Ovis aries; NCBI TaxID: 9940;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics maXis series

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-06 00:28:37	ID requested
1	2017-01-23 02:16:13	announced
⏵ 2	2024-10-22 04:32:52	announced	2024-10-22: Updated project metadata.

Karbiener M, Darnhofer B, Frisch MT, Rinner B, Birner-Gruenberger R, Gugatschka M, Comparative proteomics of paired vocal fold and oral mucosa fibroblasts. J Proteomics, 155():11-21(2017) [pubmed]

10.1016/j.jprot.2017.01.010;

curator keyword: Biological

submitter keyword: lamina propria, oral mucosa, wound healing, fibrosis,laryngology, scar, vocal folds, fibroblasts

Ruth Birner-Gruenberger
contact affiliation	Research Unit "Functional Proteomics and Metabolic Pathways", Institute of Pathology, Medical University of Graz, Stiftingtalstrasse 24, 8010 Graz, Austria; Austrian Center of Industrial Biotechnology (ACIB), Petersgasse 14, 8010 Graz, Austria, Omics Center Graz, Austria
contact email	ruth.birner-gruenberger@medunigraz.at
lab head
Barbara Darnhofer
contact affiliation	Omics Center Graz
contact email	barbara.darnhofer@klinikum-graz.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD005106

PRIDE project URI

• PRIDE
  1. PXD005106
     1. Label: PRIDE project
     2. Name: Comparative Proteomics of PairedVocal Fold and Oral Mucosa Fibroblasts