PXD005105

PXD005105 is an original dataset announced via ProteomeXchange.

Title	Enhanced limonene production in Synechococcus elongatus PCC 7942
Description	In this study, through computational modeling and synthetic biology tools, we identified and overcame a key flux controlling node in MEP-derived terpene biosynthesis in cyanobacteria. This strategy bypasses the limitation in traditional stepwise metabolic engineering, and led to significantly higher terpene productivity. Moreover, our results revealed photosynthesis limitations during high level of terpene production. We suggest that fine-tuning energy and reductant requirement could be a key factor in establishing a viable ‘non-native’ terpene sink in photosynthetic systems.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_02:05:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	XIN WANG
SpeciesList	scientific name: Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2); NCBI TaxID: 1140;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-06 00:12:43	ID requested
1	2016-12-07 07:12:41	announced
⏵ 2	2017-10-24 02:05:23	announced	Updated project metadata.

Wang X, Liu W, Xin C, Zheng Y, Cheng Y, Sun S, Li R, Zhu XG, Dai SY, Rentzepis PM, Yuan JS, Enhanced limonene production in cyanobacteria reveals photosynthesis limitations. Proc Natl Acad Sci U S A, 113(50):14225-14230(2016) [pubmed]

curator keyword: Biological

submitter keyword: limonene MEP terpene modeling ATP/NADPH

Joshua S. Yuan & Susie Y. Dai
contact affiliation	Department of Plant Pathology and Microbiology, Texas A&M University
contact email	syuan@tamu.edu
lab head
XIN WANG
contact affiliation	Texas A&M University
contact email	xwang@tamu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD005105

PRIDE project URI

• PRIDE
  1. PXD005105
     1. Label: PRIDE project
     2. Name: Enhanced limonene production in Synechococcus elongatus PCC 7942