PXD005102

PXD005102 is an original dataset announced via ProteomeXchange.

Title	Space mice re-adaption - Re-adaption on Earth after Spaceflights Affects the Mouse Liver Proteome
Description	Harsh environmental conditions including microgravity and radiation during prolonged spaceflights are known to alter hepatic metabolism. Our studies have focused on the analysis of possible changes in metabolic pathways in livers of mice which experienced 30 days of spaceflight with and without an additional re-adaption period of 7 days compared to control mice on Earth. Utilizing shotgun mass spectrometry and label-free quantification, we performed proteomic profiling to investigate mice livers from the spaceflight project “Bion-M 1”. No significant alterations in protein levels were observed between control mice liver and spaceflight mice which is possibly caused by insufficient fold change detection combined with high variances within the groups. In contrast, our results show that more than a third of the quantified protein levels are altered comparing the liver proteome of mice with and without re-adaption time after their spaceflight. Proteins related to amino acid metabolism showed higher levels after re-adaption, which may indicate higher rates of gluconeogenesis. Members of the peroxisome proliferator-activated receptor pathway reconstitute their level after 7 days due to a decrease in fold change, which indicates decreased signs of non-alcoholic fatty liver disease. Moreover, bile acid secretion regenerates on Earth due to reconstitution of related transmembrane proteins and elevated levels of the drug-metabolising enzymes belonging to the CYP superfamily decrease 7 days after the spaceflight. Thus, our study demonstrates reconstitution of pharmacological response and early signs of non-alcoholic fatty liver disease recover within 7 days, whereas glucose uptake should be monitored due to alterations in gluconeogenesis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:32:43.202.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Viktoria Anselm
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-05 05:36:49	ID requested
1	2017-08-22 07:18:11	announced
⏵ 2	2024-10-22 04:32:51	announced	2024-10-22: Updated project metadata.

10.3390/ijms18081763;

Anselm V, Novikova S, Zgoda V, Re-adaption on Earth after Spaceflights Affects the Mouse Liver Proteome. Int J Mol Sci, 18(8):(2017) [pubmed]

curator keyword: Biological

submitter keyword: metabolism,spaceflight, re-adaption, mice, liver

Victor Zgoda
contact affiliation	Department of Proteomic Research and Mass Spectrometry, Laboratory of Systems Biology, Institute of Biomedical Chemistry, Moscow, Russia
contact email	victor.zgoda@gmail.com
lab head
Viktoria Anselm
contact affiliation	Signatope GmbH, Markwiesenstr. 55 72770 Reutlingen
contact email	mail@v-anselm.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/08/PXD005102

PRIDE project URI

• PRIDE
  1. PXD005102
     1. Label: PRIDE project
     2. Name: Space mice re-adaption - Re-adaption on Earth after Spaceflights Affects the Mouse Liver Proteome