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PXD005101

PXD005101 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLipid metabolism modulation is associated with molecular injury and kidney dysfunction in a rat ischemia model
DescriptionDue to organ shortage, the transplantation community are increasingly using kidney from deceased donors such as donation after circulatory death (DCD) and donation after brain death (DBD). However, DCD donation have increased delayed graft function compared to DBD, an underline mechanism is still not well defied at the molecular level. In this study, we employed a rat model to mimic warm ischemia (45 mins) and reperfusion injury (IRI) for 4h and 24h. Apoptotic and tissue histological staining confirmed apoptosis and necrosis occurred at 4h and 24h post IRI respectively. Tissue proteome study revealed acute phase response, coagulation and complement activation and lipid X receptor activation as major pathway altered in intervention kidneys. Metabolomics follow up disclosed an increased level of lipids and fatty acids (FA). Mitochondrial function analysed by mitochondrial complex I activity and oxygen consumption and ATP levels in intervention kidney tissues were maintained 4h post IRI, but was significantly reduced 24h post IRI. Integrated proteo-metabolome analysis discovered an increased FA beta-oxidation 4h post IRI to sustain energy production. Kidney function were declined 24h post IRI indicated by increased blood creatinine and lactate levels. This study provides the frame work for the design of future metabolic intervention strategies to minimize kidney injury.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_10:06:04.545.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD005101
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterPhilip Charles
SpeciesList scientific name: Rattus rattus (Black rat); NCBI TaxID: 10117;
ModificationListOxidation
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-10-05 03:33:03ID requested
12024-10-07 10:06:09announced
Publication List
10.6019/PXD005101;
Huang H, van Dullemen LFA, Akhtar MZ, Faro ML, Yu Z, Valli A, Dona A, Th, é, z, é, nas ML, Charles PD, Fischer R, Kaisar M, Leuvenink HGD, Ploeg RJ, Kessler BM, Proteo-metabolomics reveals compensation between ischemic and non-injured contralateral kidneys after reperfusion. Sci Rep, 8(1):8539(2018) [pubmed]
10.1038/s41598-018-26804-8;
Keyword List
curator keyword: Biological
submitter keyword: Metabolomics, Lipid Metabolism,β-oxidation, Kidney Transplantation, Ischaemia Reperfusion Injury (IRI), Proteomics, Fatty Acids, GCXGC-MS
Contact List
Benedikt Kessler
contact affiliationTarget Discovery Institute, NDMRB, University of Oxford
contact emailbenedikt.kessler@ndm.ox.ac.uk
lab head
Philip Charles
contact affiliationUniversity of Oxford
contact emailphilip.charles@ndm.ox.ac.uk
dataset submitter
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Dataset FTP location
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