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PXD005096

PXD005096 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMicroproteomics application for the limited number of cells
Description Mass spectrometry (MS)-based proteomics explores in-depth proteome, however, the analysis of cell subsets, which might be minor population, is hampered due to a multistep procedure for sample preparation and purification. Along those lines, optimization of proteomics workflow toward limited number of cells is an asset. Here, we report our workflow of microproteomics, proteomics for a limited number of cells: a microfluidic-chip based cell sorter was applied for a damage-free cell sorting, and each 100 or 1000 THP-1 cells were collected in hydrophilic or albumin-coated microtubes. Following in-solution enzymatic digestions, peptide mixtures were purified and applied to nanoLC-MS (Orbitrap Elite) analysis with 3 hr gradient. Our method enabled proteome mapping from 1000-sorted cells, with a similar in-depth map from 1 μg of cell lysate. This approach would be useful for cell subsets and limited materials such as circulating tumor cells, inflammatory cells in cancer tissues and clinical materials.
HostingRepositoryjPOST
AnnounceDate2017-07-18
AnnouncementXMLSubmission_2022-09-18_03:22:51.247.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKie Kasuga
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; unknown modification
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-10-04 22:54:26ID requested
12017-07-18 06:53:51announced
22017-07-31 00:41:19announcedUpdated PubMed.
32017-07-31 00:44:31announcedUpdated PubMed.
42018-09-18 22:56:23announcedUpdated CV, FTP location and Modification list.
52021-02-04 16:44:24announced2021-02-04: Updated FTP location.
62022-09-18 03:22:52announced2022-09-18: Updated FTP location.
Publication List
Kasuga K, Katoh Y, Nagase K, Igarashi K, Microproteomics with microfluidic-based cell sorting: Application to 1000 and 100 immune cells. Proteomics, 17(13-14):(2017) [pubmed]
Keyword List
submitter keyword: THP-1, shotgun proteomics, Cell-sorting, microproteomics
Contact List
Kie Kasuga
lab head
Kie Kasuga
contact affiliationKanazawa University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000161/