PXD005096
PXD005096 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Microproteomics application for the limited number of cells |
| Description | Mass spectrometry (MS)-based proteomics explores in-depth proteome, however, the analysis of cell subsets, which might be minor population, is hampered due to a multistep procedure for sample preparation and purification. Along those lines, optimization of proteomics workflow toward limited number of cells is an asset. Here, we report our workflow of microproteomics, proteomics for a limited number of cells: a microfluidic-chip based cell sorter was applied for a damage-free cell sorting, and each 100 or 1000 THP-1 cells were collected in hydrophilic or albumin-coated microtubes. Following in-solution enzymatic digestions, peptide mixtures were purified and applied to nanoLC-MS (Orbitrap Elite) analysis with 3 hr gradient. Our method enabled proteome mapping from 1000-sorted cells, with a similar in-depth map from 1 μg of cell lysate. This approach would be useful for cell subsets and limited materials such as circulating tumor cells, inflammatory cells in cancer tissues and clinical materials. |
| HostingRepository | jPOST |
| AnnounceDate | 2017-07-18 |
| AnnouncementXML | Submission_2022-09-18_03:22:51.247.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Kie Kasuga |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; unknown modification |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2016-10-04 22:54:26 | ID requested | |
| 1 | 2017-07-18 06:53:51 | announced | |
| 2 | 2017-07-31 00:41:19 | announced | Updated PubMed. |
| 3 | 2017-07-31 00:44:31 | announced | Updated PubMed. |
| 4 | 2018-09-18 22:56:23 | announced | Updated CV, FTP location and Modification list. |
| 5 | 2021-02-04 16:44:24 | announced | 2021-02-04: Updated FTP location. |
| ⏵ 6 | 2022-09-18 03:22:52 | announced | 2022-09-18: Updated FTP location. |
Publication List
| Kasuga K, Katoh Y, Nagase K, Igarashi K, Microproteomics with microfluidic-based cell sorting: Application to 1000 and 100 immune cells. Proteomics, 17(13-14):(2017) [pubmed] |
Keyword List
| submitter keyword: THP-1, shotgun proteomics, Cell-sorting, microproteomics |
Contact List
| Kie Kasuga | |
|---|---|
| lab head | |
| Kie Kasuga | |
| contact affiliation | Kanazawa University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000161/ |
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