PXD005095

PXD005095 is an original dataset announced via ProteomeXchange.

Title	A role for barley calcium-dependent protein kinase CPK2a in the response to drought
Description	Increasing the drought tolerance of crops is one of the most challenging goals in plant breeding. To improve crop productivity during periods of water deficit, it is essential to understand the complex regulatory pathways that adapt plant metabolism to environmental conditions. Among various plant hormones and second messengers, calcium ions are known to be involved in drought stress perception and signaling. Plants have developed specific calcium-dependent protein kinases that convert calcium signals into phosphorylation events. In this study we attempted to elucidate the role of a calcium-dependent protein kinase in the drought stress response of barley (Hordeum vulgare L.), one of the most economically important crops worldwide. The ongoing barley genome project has provided useful information about genes potentially involved in the drought stress response, but information on the role of calcium-dependent kinases is still limited. We found that the gene encoding the calcium-dependent protein kinase HvCPK2a was significantly upregulated in response to drought. To better understand the role of HvCPK2a in drought stress signaling, we generated transgenic Arabidopsis plants that overexpressed the corresponding coding sequence. Overexpressing lines displayed drought sensitivity, reduced nitrogen balance index, an increase in total chlorophyll content and decreased relative water content. In addition, in vitro kinase assay experiments combined with mass spectrometry allowed HvCPK2a autophosphorylation sites to be identified. Our results suggest that HvCPK2a is a dual-specificity calcium-dependent protein kinase that functions as a negative regulator of the drought stress response in barley.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:32:55.564.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maciej Janicki
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Nicotiana benthamiana; NCBI TaxID: 4100; scientific name: Hordeum vulgare (Barley); NCBI TaxID: 4513;
ModificationList	ubiquitination signature dipeptidyl lysine; phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-04 05:35:21	ID requested
1	2016-12-23 07:04:19	announced
2	2017-10-24 03:40:17	announced	Updated project metadata.
⏵ 3	2024-10-22 04:32:56	announced	2024-10-22: Updated project metadata.

Cie, ś, la A, Mitu, ł, a F, Misztal L, Fedorowicz-Stro, ń, ska O, Janicka S, Tajdel-Zieli, ń, ska M, Marczak M, Janicki M, Ludwik, ó, w A, Sadowski J, A Role for Barley Calcium-Dependent Protein Kinase CPK2a in the Response to Drought. Front Plant Sci, 7():1550(2016) [pubmed]

curator keyword: Biological

submitter keyword: drought stress, mass spectrometry,calcium-dependent kinase, phosphorylation, Hordeum vulgare L., post-translational modification (PTM)

Agnieszka Ludwików
contact affiliation	Biotechnology Department, Faculty of Biology, Adam Mickiewicz University, Umultowska Street 89, 61-614 Poznań, Poland
contact email	ludwika@amu.edu.pl
lab head
Maciej Janicki
contact affiliation	UAM
contact email	mjanicki@amu.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD005095

PRIDE project URI

• PRIDE
  1. PXD005095
     1. Label: PRIDE project
     2. Name: A role for barley calcium-dependent protein kinase CPK2a in the response to drought