PXD005093

PXD005093 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteome of Tanshinone IIA treatment in Human gastric cell line AGS
Description	Gastric cancer is one of the world common causes of cancer death. Many people have suffered, but yet therapeutic methods found. May studies have showedthat Tanshinone IIA, a diterpenequinone, which extracted from the traditional herbal medicine Danshen (Salvia miltiorrhiza),has potential against many kind of cancer types. Our previous studies have demonstrated TIIA can kill gastric cancer AGS cells, but the response signalling is still unclear. Therefore, we used the time-dependent phosphoproteomic approach to reveal the regulatory effects of TIIA in AGS cells. Our results showed that a total of 1092 phosphoprotiens and 3332 phosphopeptides were identified in 3615 phosphorylation sites and 349 phosphosites corresponding to 220 phosphorylated proteins were significantly regulated. Furthermore, by using networkand functionalenrichmentanalyses, we found that TIIA regulated several cellular processesingastric cancer cells, such astranscription mRNA processing, DNA damage and protein unfolding response. Finally, we further validated that TIIA caused protein unfolding response and DNA damage via inducing ROS production. These findings not only uncover the molecular mechanisms mediated by TIIA but also contribute to the development of gastric cancer therapy.
HostingRepository	PRIDE
AnnounceDate	2020-03-18
AnnouncementXML	Submission_2020-03-18_05:21:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chieh-Fan Yin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-04 02:04:37	ID requested
⏵ 1	2020-03-18 05:21:44	announced

Yin CF, Kao SC, Hsu CL, Chang YW, Cheung CHY, Huang HC, Juan HF, Phosphoproteome Analysis Reveals Dynamic Heat Shock Protein 27 Phosphorylation in Tanshinone IIA-Induced Cell Death. J Proteome Res, 19(4):1620-1634(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: Human gastric cell line AGS, Tanshinone IIA, phosphoproteome, Dimethyl labeling, Strong cation-exchange chromatography fractionation, Hydroxy acid-modified metal oxide chromatography, LC-MS/MS

Hsueh-Fen Juan
contact affiliation	Institute of Molecular and Cellular Biology, National Taiwan University, Taipei, Taiwan
contact email	yukijuan@gmail.com
lab head
Chieh-Fan Yin
contact affiliation	National Taiwan University
contact email	d03b43004@ntu.edu.tw
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD005093

PRIDE project URI

• PRIDE
  1. PXD005093
     1. Label: PRIDE project
     2. Name: Phosphoproteome of Tanshinone IIA treatment in Human gastric cell line AGS