PXD005063

PXD005063 is an original dataset announced via ProteomeXchange.

Title	Identification of human membrane proteins
Description	Typically, integral membrane and membrane associate proteins are underrepresented in proteomic experiments due to their low abundance and solubility during sample preparation. We collected two fractions upon cell lysis, one comprising the insoluble membrane fraction the other the soluble cytosolic fraction. Using detergents and additives we were able to release the proteins from the membrane fraction and subsequently perform trypsin digestion for analysis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:32:52.350.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Schmidt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-29 03:05:21	ID requested
1	2019-02-25 01:24:45	announced
⏵ 2	2024-10-22 04:33:00	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.8b00819;

Ammar C, Berchtold E, Csaba G, Schmidt A, Imhof A, Zimmer R, Multi-Reference Spectral Library Yields Almost Complete Coverage of Heterogeneous LC-MS/MS Data Sets. J Proteome Res, 18(4):1553-1566(2019) [pubmed]

curator keyword: Biological

submitter keyword: membrane proteins, quantitatie proteomics

Axel Imhof
contact affiliation	Protein Analysis Unit ZfP LMU Munich BioMedical Center Grosshaderner Strasse 9 82152 Planegg-Martinsried Tel. +49 89 2180 71804
contact email	imhof@lmu.de
lab head
Andreas Schmidt
contact affiliation	CECAD, University of Cologne, AG Krüger
contact email	A.Schmidt@med.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD005063

PRIDE project URI

• PRIDE
  1. PXD005063
     1. Label: PRIDE project
     2. Name: Identification of human membrane proteins