PXD005058

PXD005058 is an original dataset announced via ProteomeXchange.

Title	Proteomics exploiting modular baits with different subcellular locations reveal hydrogen peroxide-inducible clone 5 protein (HIC-5) and a glucocorticoid receptor variant as novel testin partners
Description	The LIM domain and tumour suppressor protein Testin (Tes) is downregulated in a variety of human tumours and tumour cell lines. Depending on its conformation, Tes localises to stress fibres and focal adhesions where it forms protein complexes with other members of the actin cytoskeleton, such as zyxin and VASP, and thereby influences cellular processes like cell migration, adhesion or spreading. Tes is a modular protein and Tes variants lacking specific domains, localize to different locations in cells. To better understand the molecular basis of its function, we utilized an interaction proteomics approach combined with pathway analysis. This revealed proteins present in complexes in which Tes participates as a function of its modular structure as well as novel Tes interaction partners. We demonstrate that Tes interacts with a short isoform of the glucocorticoid receptor (GR), independently of the conventional full length GRα, a transcription factor important in regulating cellular metabolism and immune function. Tes also interacts with the focal adhesion protein and GR-coactivator Hic-5. In addition, we found that upon overexpression, Tes and Hic-5 induce opposite effects on cell spreading on a fibronectin matrix.
HostingRepository	PRIDE
AnnounceDate	2018-10-26
AnnouncementXML	Submission_2018-10-26_13:37:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	An Staes
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-28 08:48:13	ID requested
⏵ 1	2018-10-26 13:37:09	announced

Sala S, Catillon M, Hadzic E, Schaffner-Reckinger E, Van Troys M, Ampe C, The PET and LIM1-2 domains of testin contribute to intramolecular and homodimeric interactions. PLoS One, 12(5):e0177879(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Testin,IP,Proteomics, LC-MS/MS, iMixPro, GR, HIC-5

Christophe Ampe
contact affiliation	Department of Biochemistry, Ghent University, Belgium
contact email	christophe.ampe@ugent.be
lab head
An Staes
contact affiliation	Medical Protein Chemistry
contact email	an.staes@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD005058

PRIDE project URI

• PRIDE
  1. PXD005058
     1. Label: PRIDE project
     2. Name: Proteomics exploiting modular baits with different subcellular locations reveal hydrogen peroxide-inducible clone 5 protein (HIC-5) and a glucocorticoid receptor variant as novel testin partners