PXD005056

PXD005056 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis (LC-MS/MS) of Pseudomonas pseudoalcaligenes CECT5344 in response to industrial cyanide-containing wastewaters
Description	Biological treatments to degrade cyanide have shown to be a powerful technology for cyanide removal from industrial wastewaters. It has been previously demonstrated that the alkaliphilic bacterium Pseudomonas pseudoalcaligenes CECT5344 is able to use free cyanide and several metal−cyanide complexes as the sole nitrogen source. The strain CECT5344 has been used for detoxification of the different chemical forms of cyanide that are present in alkaline wastewaters from the electroplating process of the jewelry industry. This liquid residue also contains large concentrations of metals like iron, copper and zinc, making this wastewater even more toxic. A proteomic analysis by LC-MS/MS has been applied to elucidate the molecular mechanisms involved in this bioremediation process in P. pseudoalcaligenes CECT5344. Among others, different proteins related to cyanide and cyanate assimilation, as well as other proteins involved in transport and resistance to metals were induced by the cyanide-containing jewelry residue have been identified.
HostingRepository	PRIDE
AnnounceDate	2017-03-08
AnnouncementXML	Submission_2017-03-08_08:22:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Victor M. Luque-Almagro
SpeciesList	scientific name: Pseudomonas pseudoalcaligenes CECT 5344; NCBI TaxID: 1182590;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-28 05:35:01	ID requested
⏵ 1	2017-03-08 08:22:14	announced

Ib, á, ñ, ez MI, Cabello P, Luque-Almagro VM, S, á, ez LP, Olaya A, S, á, nchez de Medina V, Luque de Castro MD, Moreno-Vivi, á, n C, Rold, á, n MD, Quantitative proteomic analysis of Pseudomonas pseudoalcaligenes CECT5344 in response to industrial cyanide-containing wastewaters using Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS). PLoS One, 12(3):e0172908(2017) [pubmed]

submitter keyword: Biodegradation, cyanide, heavy metals, jewelry residue, Pseudomonas, Proteomics

Mª Dolores Roldán Ruíz
contact affiliation	Departamento de Bioquímica y Biología Molecular. Edificio Severo Ochoa. Campus de Rabanales. Universidad de Córdoba. Córdoba, 14071. Spain
contact email	bb2rorum@uco.es
lab head
Victor M. Luque-Almagro
contact affiliation	Universidad de Córdoba
contact email	b42lualv@uco.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/03/PXD005056

PRIDE project URI

• PRIDE
  1. PXD005056
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis (LC-MS/MS) of Pseudomonas pseudoalcaligenes CECT5344 in response to industrial cyanide-containing wastewaters