PXD005054

PXD005054 is an original dataset announced via ProteomeXchange.

Title	Combinatory shotgun proteomics and shotgun eicosadomics study of platelets activated by calcium mobilization - secreted proteins of platelets stimulated for 3h with ionomycin
Description	Platelets are involved in relevant physiological and pathophysiological processes such as hemostasis and tumor progression. These anucleate blood particles exert their functions essentially by releasing effector molecules such as proteins from intracellular granules and by generating and secreting specific lipids. In order to contribute to a systematic proteomics and eicosadomics analysis of platelets activated by calcium mobilization, we have investigated proteins and eicosanoids in lysates and secretomes of platelets activated with ionomycin. To this end, self-established shotgun proteomics and shotgun lipidomics platforms were used. Applying an FDR of less than 0.01 at both, peptide and protein level, a total of 2543 protein groups was identified. Several regulatory effects concerning proteins were determined, including protein secretion, degradation, as well as protein synthesis. Incorporation of 35S-methionine in platelet proteins determined by 2D-PAGE confirmed de-novo protein synthesis. Moreover, we were able to determine a complex set of eicosanoids synthesized by activated platelets, including 15S-HETE, prostaglandin E2 and pro-survival factors such as 12-HETE, as well as eicosanoids previously undescribed to have platelet functions, such as 9-HETE and 11-HETE. Proteomics data complemented the findings from lipidomics experiments and supported the generation of a map indicating pathways leading to eicosanoid production in platelets upon calcium mobilization.
HostingRepository	PRIDE
AnnounceDate	2022-03-01
AnnouncementXML	Submission_2022-03-01_08:06:38.925.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005054
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christopher Gerner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	residues isobaric at 128.058578 Da; Oxidation; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-28 02:57:07	ID requested
⏵ 1	2022-03-01 08:06:41	announced

Meier SM, Kreutz D, Winter L, Klose MHM, Cseh K, Weiss T, Bileck A, Alte B, Mader JC, Jana S, Chatterjee A, Bhattacharyya A, Hejl M, Jakupec MA, Heffeter P, Berger W, Hartinger CG, Keppler BK, Wiche G, Gerner C, An Organoruthenium Anticancer Agent Shows Unexpected Target Selectivity For Plectin. Angew Chem Int Ed Engl, 56(28):8267-8271(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Human platelets, activation by calcium mobilization, secretomes, lysates, mass spectrometry, shotgun proteomics and eicosadomics

Christopher Gerner
contact affiliation	University of Vienna, Faculty of Chemistry, Department of Analytical Chemistry
contact email	christopher.gerner@univie.ac.at
lab head
Christopher Gerner
contact affiliation	University of Vienna
contact email	christopher.gerner@univie.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005054
     1. Label: PRIDE project
     2. Name: Combinatory shotgun proteomics and shotgun eicosadomics study of platelets activated by calcium mobilization - secreted proteins of platelets stimulated for 3h with ionomycin