⮝ Full datasets listing

PXD005048

PXD005048 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic characterization of exosomes derived from malaria-infected reticulocytes, in a murine malaria model
DescriptionReticulocyte-derived exosomes (rex) are 30-100 nm membrane vesicles of endocytic origin released during the maturation of reticulocytes to erythrocytes upon fusion of multivesicular bodies with the plasma membrane. We previously found that immunization of mice with rex from BALB/c mice infected with the reticulocyte-prone non-lethal P. yoelii 17X malaria strain (rexPy) in combination with CpG-ODN promoted survival and long lasting protection of mice subsequently challenged with a lethal strain. Here, we show that rexPy-mediated protection is completely lost in splenectomized animals and such protection is achieved after passive transfer of splenocytes obtained from animals immunized with rexPy+CpG. Notably, rexPy immunization of mice induced non-exhausted memory T cell expansion with effector phenotype. Proteomics analysis of rexPy confirmed their reticulocyte origin and demonstrated the presence of parasite antigens. Our studies thus prove, for what we believe is the first time, that rex from reticulocyte-prone malarial infections are able to induce non-exhausted splenic long-lasting memory responses. In order to translate these results to humans, in vitro experiments with spleen cells of human transplantation donors were performed. Human splenocytes were able to actively capture plasma45 derived exosomes from patients infected with Plasmodium vivax (exPv) and stimulation of spleen cells with exPv for 72h lead to an increase in the number of T cells. All together, these data further support the value of reticulocyte-derived exosomes as a potential novel vaccine and platform against malaria.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:29:04.391.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterArmando Neto
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Plasmodium yoelii; NCBI TaxID: 5861;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-09-28 02:14:22ID requested
12018-10-26 13:39:32announced
22024-10-22 04:29:07announced2024-10-22: Updated project metadata.
Publication List
Mart, í, n-Jaular L, de Menezes-Neto A, Mongui, ó, -Tortajada M, Elizalde-Torrent A, D, í, az-Varela M, Fern, á, ndez-Becerra C, Borras FE, Montoya M, Del Portillo HA, Spleen-Dependent Immune Protection Elicited by CpG Adjuvanted Reticulocyte-Derived Exosomes from Malaria Infection Is Associated with Changes in T cell Subsets' Distribution. Front Cell Dev Biol, 4():131(2016) [pubmed]
10.3389/fcell.2016.00131;
Keyword List
curator keyword: Biomedical
submitter keyword: reticulocytes, malaria,exosomes, spleen
Contact List
Hernando Antonio del Portillo
contact affiliationICREA at ISGlobal Institute for Glbal Health, Hospital Clinic - Universitat de Barcelona, Barcelona, Spain and IGTP Institut d'Investigació Germans Trias i Pujol, Badalona, Spain
contact emailhernandoa.delportillo@isglobal.org
lab head
Armando Neto
contact affiliationCPqRR-FIOCRUZ
contact emailarmandomenezes@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD005048
PRIDE project URI
Repository Record List
[ + ]