PXD005043

PXD005043 is an original dataset announced via ProteomeXchange.

Title	Discovery of Novel Antimicrobial Peptides from Varanus komodoensis (Komodo dragon) by Large Scale Analyses and De Novo Assisted Sequencing using Electron Transfer Dissociation Mass Spectrometry
Description	We have employed a custom bioprospecing approach to identify cationic antimicrobial peptides from Komodo dragon plasma. Through these analyses we identified forty eight novel potential cationic antimicrobial peptides, with all but one of the identified peptides being derived from histone proteins. The antimicrobial effectiveness of eight of these peptides was evaluated against Pseudomonas aeruginosa 9027 and Staphylococcus aureus 25983, with seven peptides exhibiting antimicrobial activity against both microbes, and one only showing significant potency against Pseudomonas aeruginosa 9027. This study demonstrates the power and promise of our bioprospecting approach to CAMP discovery and it reveals the presence of a plethora of novel histone-derived antimicrobial peptides in the plasma of the dragon. These findings may have broader implications regarding the role that intact histones and histone-derived peptides play in defending the host from infection.
HostingRepository	PRIDE
AnnounceDate	2017-02-08
AnnouncementXML	Submission_2017-02-08_06:23:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barney Bishop
SpeciesList	scientific name: Varanus komodoensis; NCBI TaxID: 61221;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-27 03:30:13	ID requested
⏵ 1	2017-02-08 06:23:19	announced

Bishop BM, Juba ML, Russo PS, Devine M, Barksdale SM, Scott S, Settlage R, Michalak P, Gupta K, Vliet K, Schnur JM, van Hoek ML, Discovery of Novel Antimicrobial Peptides from Varanus komodoensis (Komodo Dragon) by Large-Scale Analyses and De-Novo-Assisted Sequencing Using Electron-Transfer Dissociation Mass Spectrometry. J Proteome Res, 16(4):1470-1482(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Komodo, plasma, antimicrobial peptides, ETD, MS/MS

Barney Bishop
contact affiliation	George Mason University, Department of Chemistry and Biochemistry, Bishop lab
contact email	bbishop1@gmu.edu
lab head
Barney Bishop
contact affiliation	George Mason University
contact email	bbishop1@gmu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD005043

PRIDE project URI

• PRIDE
  1. PXD005043
     1. Label: PRIDE project
     2. Name: Discovery of Novel Antimicrobial Peptides from Varanus komodoensis (Komodo dragon) by Large Scale Analyses and De Novo Assisted Sequencing using Electron Transfer Dissociation Mass Spectrometry