PXD005031

PXD005031 is an original dataset announced via ProteomeXchange.

Title	A quantitative map of proteome dynamics during neuronal differentiation
Description	Neuronal differentiation is a multistep process that shapes and re-shapes neurons by progressing through several typical stages, including axon outgrowth, dendritogenesis and synapse formation. To systematically profile protein expression during neuronal differentiation we have used cultured hippocampal neurons at different developmental stages in combination with triplex stable isotope dimethyl labeling technique coupled to high-resolution tandem mass spectrometry (LC-MS/MS). In total >1,500 proteins show more than two-fold expression changes during the different developmental steps, indicating extensive remodeling of the neuron proteome during differentiation. To demonstrate the strength of our resource, we focused on neural cell adhesion molecule 1 (NCAM1) as a regulator for dendritic outgrowth during neuronal development. The transmembrane isoform of NCAM1, NCAM180, is strongly upregulated during dendrite outgrowth, highly enriched in dendritic growth cones and interacts with a large variety of actin binding proteins. Inducing actin polymerization rescues the NCAM1 knockdown phenotype, suggesting that NCAM180 stimulates dendritic arbor development by promoting actin filament growth at the dendritic growth cone. Thus, our quantitative map of neuronal proteome dynamics will serve as a rich resource for further analyses of neurodevelopmental regulated processes.
HostingRepository	PRIDE
AnnounceDate	2017-01-17
AnnouncementXML	Submission_2017-06-09_08:54:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Frese
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-26 00:31:29	ID requested
1	2017-01-17 03:56:48	announced
⏵ 2	2017-06-09 08:54:14	announced	Updated publication reference for PubMed record(s): 28178528.

Frese CK, Mikhaylova M, Stucchi R, Gautier V, Liu Q, Mohammed S, Heck AJR, Altelaar AFM, Hoogenraad CC, Quantitative Map of Proteome Dynamics during Neuronal Differentiation. Cell Rep, 18(6):1527-1542(2017) [pubmed]

curator keyword: Biological

submitter keyword: Neuronal differentiation, neuronal development, dendrite outgrowth, adhesion molecules, NCAM1, adhesion molecule, proteome, quantitative proteomics, stable isotope dimethyl labeling, mass spectrometry

AF Maarten Altelaar
contact affiliation	Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
contact email	m.altelaar@uu.nl
lab head
Christian Frese
contact affiliation	CECAD Research Center Cologne
contact email	cfrese@uni-koeln.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD005031

PRIDE project URI

• PRIDE
  1. PXD005031
     1. Label: PRIDE project
     2. Name: A quantitative map of proteome dynamics during neuronal differentiation