PXD005028

PXD005028 is an original dataset announced via ProteomeXchange.

Title	SpaP and SpaR interaction partner identification
Description	Bacterial type III protein secretion systems inject effector proteins into eukaryotic host cells in order to promote survival and colonization of Gram-negative pathogens and symbionts. Secretion across the bacterial cell envelope and injection into host cells is facilitated by a so-called needle complex. Its small hydrophobic export apparatus components SpaP and SpaR were shown to nucleate assembly of the needle complex and to form the central “cup” substructure of a Salmonella Typhimurium secretion system, however, the in vivo placement of these components in the needle complex and their function during the secretion process remained poorly defined. Here we show that a SpaP pentamer forms a 15 Å wide pore and present a detailed map of SpaP interactions with the export apparatus components SpaQ, SpaR, and SpaS. We further demonstrate the formation of a continuous conduit for substrate translocation and injection by intimate interactions of the periplasmic domains of SpaP and SpaR with the inner rod protein PrgJ, refine the current view of export apparatus assembly and consolidate transmembrane topology models for SpaP and SpaR.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:49:10.373.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nicolas Nalpas
SpeciesList	scientific name: Salmonella enterica subsp. enterica serovar Typhimurium; NCBI TaxID: 90371;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-23 04:26:19	ID requested
1	2016-11-29 06:54:36	announced
2	2016-12-06 01:33:11	announced	Updated publication reference for DOI(s): 10.1371/journal.ppat.1006071.
⏵ 3	2023-11-14 08:49:10	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: SpaR, SpaP,T3SS, photocrosslinking

Samuel Wagner
contact affiliation	Eberhard Karls University Tübingen Interfaculty Institute of Microbiology and Infection Medicine (IMIT)
contact email	Samuel.Wagner@med.uni-tuebingen.de
lab head
Nicolas Nalpas
contact affiliation	Tuebingen University
contact email	nalpas.nicolas@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/11/PXD005028

PRIDE project URI

• PRIDE
  1. PXD005028
     1. Label: PRIDE project
     2. Name: SpaP and SpaR interaction partner identification