PXD005026

PXD005026 is an original dataset announced via ProteomeXchange.

Title	A heterochromatin-dependent transcription machinery drives piRNA expression
Description	Nuclear small RNA pathways safeguard genome integrity by establishing transcription-repressing heterochromatin at transposable elements. This inevitably also targets the transposon-rich source loci of the small RNAs themselves. How small RNA source loci are efficiently transcribed while transposon promoters are potently silenced, is not understood. Here, we show that transcription of Drosophila piRNA clusters—small RNA source loci in animal gonads—is enforced through RNA Polymerase II pre-initiation complex formation within repressive heterochromatin. This is accomplished through the TFIIA-L paralog Moonshiner, which is recruited to piRNA clusters via the Heterochromatin Protein-1 variant Rhino. Moonshiner triggers transcription initiation within piRNA clusters by recruiting the TATA box-binding protein (TBP)-related factor TRF2, an animal TFIID core variant. Thus, transcription of heterochromatic small RNA source loci relies on direct recruitment of the core transcriptional machinery to DNA via histone marks rather than sequence motifs, a concept that we argue is a recurring theme in evolution.
HostingRepository	PRIDE
AnnounceDate	2017-08-07
AnnouncementXML	Submission_2017-09-12_02:26:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Imre
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	methylthiolated residue; phosphorylated residue; monohydroxylated residue; acetylated residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-23 00:20:08	ID requested
1	2017-08-07 05:48:14	announced
⏵ 2	2017-09-12 02:26:29	announced	Updated publication reference for PubMed record(s): 28847004.

Andersen PR, Tirian L, Vunjak M, Brennecke J, A heterochromatin-dependent transcription machinery drives piRNA expression. Nature, 549(7670):54-59(2017) [pubmed]

curator keyword: Biological

submitter keyword: Drosophila, Ovary, piRNA, transcription

Julius Fabian Brennecke
contact affiliation	IMBA - Institute of Molecular Biotechnology Dr. Bohrgasse 3 1030 Vienna Austria
contact email	julius.brennecke@imba.oeaw.ac.at
lab head
Richard Imre
contact affiliation	IMBA - Institute of Molecular Biotechnology
contact email	imre@imp.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/08/PXD005026

PRIDE project URI

• PRIDE
  1. PXD005026
     1. Label: PRIDE project
     2. Name: A heterochromatin-dependent transcription machinery drives piRNA expression