PXD005015

PXD005015 is an original dataset announced via ProteomeXchange.

Title	Identification of the circadian ubiquitylated proteome of Drosophila melanogaster.
Description	Circadian clocks have evolved as time-measuring molecular devices to help organisms adapt their physiology to daily changes in light and temperature. Cycling transcription has been long hypothesized to account for the wealth of rhythmic protein abundance. However, cyclic degradation signals such as ubiquitylation could shape the rhythmic protein landscape as well. In order to document the circadian ubiquitylated proteome of Drosophila melanogaster, we took advantage of a new means of Ub purification based on in vivo biotinylation of AviTag-tagged ubiquitin by the BirA protein, the bioUb system. NeutrAvidin-bound fractions of head lysates were collected at four circadian times six hours apart and proteins were identified and quantified using a proteomic-based approach.
HostingRepository	PRIDE
AnnounceDate	2018-05-22
AnnouncementXML	Submission_2018-05-22_00:48:05.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005015
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Cornu
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	Oxidation; Carbamidomethyl
Instrument	TripleTOF 4600

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-21 06:16:06	ID requested
⏵ 1	2018-05-22 00:48:06	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Circadian rhythm, ubiquitylation, posttranslational regulation, proteomics

David Cornu
contact affiliation	SICaPS, Institute of Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Univ. Paris-Saclay 91198 Gif-sur-Yvette Cedex, France
contact email	david.cornu@i2bc.paris-saclay.fr
lab head
David Cornu
contact affiliation	SICaPS, Institute of Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Univ. Paris-Saclay 91198 Gif-sur-Yvette Cedex, France
contact email	david.cornu@i2bc.paris-saclay.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD005015

PRIDE project URI

• PRIDE
  1. PXD005015
     1. Label: PRIDE project
     2. Name: Identification of the circadian ubiquitylated proteome of Drosophila melanogaster.