PXD005006

PXD005006 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of enteroid cultures skewed towards development of specific epithelial lineages
Description	Recently, three-dimensional small intestinal organoids (enteroids) have been developed from cultures of intestinal stem cells which differentiate in vitro to generate all the differentiated epithelial cell types associated with the intestine and mimic the structural properties of the intestine observed in vivo. Small-molecule drug treatment can skew organoid epithelial cell differentiation towards particular lineages, and these skewed enteroids may provide useful tools to study specific epithelial cell populations, such as goblet and Paneth cells. However, the use and characterisation of enteroid models has not yet been fully explored, such that the extent to which differentiated epithelial cell populations in these skewed enteroids represent their in vivo counterparts is not fully understood. In this study, we have performed label-free quantitative proteomics to determine whether skewing murine enteroid cultures towards the goblet or Paneth cell lineages results in changes in abundance of proteins associated with these cell lineages in vivo. Our data confirm that skewed enteroids recapitulate important features of the in vivo gut environment, confirming that they can serve as useful models for the investigation of normal and disease processes in the intestine. Furthermore, by comparison of our mass spectrometry data with histology data contained within the Human Protein Atlas, we identify putative novel markers for goblet and Paneth cells.
HostingRepository	PRIDE
AnnounceDate	2019-01-11
AnnouncementXML	Submission_2019-01-11_01:20:03.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005006
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Stuart Armstrong
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-20 05:26:33	ID requested
⏵ 1	2019-01-11 01:20:04	announced

Luu L, Matthews ZJ, Armstrong SD, Powell PP, Wileman T, Wastling JM, Coombes JL, Proteomic Profiling of Enteroid Cultures Skewed toward Development of Specific Epithelial Lineages. Proteomics, 18(16):e1800132(2018) [pubmed]

curator keyword: Biological

submitter keyword: differentiation

mass spectrometry

gastrointestinal system

stem cells

Julian Hiscox
contact affiliation	Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool
contact email	julianh@liverpool.ac.uk
lab head
Stuart Armstrong
contact affiliation	Infection Biology
contact email	sarmstro@liv.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005006
     1. Label: PRIDE project
     2. Name: Proteomic profiling of enteroid cultures skewed towards development of specific epithelial lineages