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PXD005004

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2018-01-10
  • AnnouncementXML: Submission_2018-01-10_04:46:10.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: James Wright
  • Title: C. rodentium remodels metabolism in IEC
  • Description: The mechanisms by which the mouse pathogen Citrobacter rodentium triggers effacement of the brush border microvilli, colitis, hyperplasia and dysbiosis remain poorly understood. We investigated the impact of C. rodentium infection on the proteomic and metabolic landscapes of intestinal epithelial cells (IECs) in vivo using isobaric labeling proteomics and targeted metabolomics. We found that infection depletes proteins involved in butyrate uptake, glycolysis, TCA cycle, lipid metabolism and oxidative phosphorylation with aparallel, increased production of creatine/phosphocreatine and cholesterol. The evolving ecological niche within the infected gut was concomitant with a reduction in butyrate-producing commensal bacteria and expansion of Proteobacteria that can metabolize cholesterol. These changes coincide with the modulation of IEC transcription factors by C. rodentium, specifically phosphorylation of Kdm5a, demethylation of histone H3 (Lys-4) and cleavage of Srebp2. Taken together our results show that whilst engaging with the host in a race to control innate immune responses, C. rodentium and the changing microbiota shape the metabolism flow in IECs.
  • SpeciesList: scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Citrobacter rodentium; NCBI TaxID: 67825;
  • ModificationList: phosphorylated residue; iodoacetamide derivatized residue
  • Instrument: Orbitrap Fusion

Dataset History

VersionDatetimeStatusChangeLog Entry
02016-09-20 03:33:20ID requested
12018-01-10 04:46:11announced

Publication List

  1. Berger CN, Crepin VF, Roumeliotis TI, Wright JC, Carson D, Pevsner-Fischer M, Furniss RCD, Dougan G, Dori-Bachash M, Yu L, Clements A, Collins JW, Elinav E, Larrouy-Maumus GJ, Choudhary JS, Frankel G, Citrobacter rodentium Subverts ATP Flux and Cholesterol Homeostasis in Intestinal Epithelial Cells In Vivo. Cell Metab, 26(5):738-752.e6(2017) [pubmed]

Keyword List

  1. submitter keyword: Mouse, Colon, TMT, LC-MSMS, Citrobacter Rodentium, Phosphorylation

Contact List

    Jyoti Choudhary
    • contact affiliation: Wellcome Trust Sanger Institute
    • contact email: jc4@sanger.ac.uk
    • lab head:
    James Wright
    • contact affiliation: Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute
    • contact email: jw13@sanger.ac.uk
    • dataset submitter:

Full Dataset Link List

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