PXD005002

PXD005002 is an original dataset announced via ProteomeXchange.

Title	Towards a comprehensive targeted proteogenomic assay repository for the liquid fraction of sheep blood
Description	This dataset presents the results of protein sample preparation strategies for enriching a novel encyclopaedic peptide spectral library using the liquid fraction of sheep blood. The method used showed the capability of simultaneously detecting hundreds of key proteins in the liquid fraction of blood (circulating acellular proteome) using sheep as a model by: a) Prototyping of the circulating acellular proteome of healthy sheep using serum b) A comprehensive analysis of fractions of acetone precipitated sheep serum and plasma; c) A comprehensive analysis of fractions of partial organic precipitation of sheep serum and plasma proteins using acetonitrile; d) Combinatorial peptide ligand library protein enrichment of sheep serum and plasma; e) Off-gel fractionation of serum proteins; f) Extraction of protein data from ex-diagnostic sheep serum; g) Derivation of peptide data from serum and plasma of endotoxin treated sheep.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:28:30.590.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005002
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Saul Chemonges
SpeciesList	scientific name: Ovis aries; NCBI TaxID: 9940;
ModificationList	Gln->pyro-Glu; Glu->pyro-Glu; Oxidation; Carbamidomethyl; Deamidated
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-20 02:24:32	ID requested
⏵ 1	2024-10-07 13:28:33	announced

Chemonges S, Shekar K, Tung JP, Dunster KR, Diab S, Platts D, Watts RP, Gregory SD, Foley S, Simonova G, McDonald C, Hayes R, Bellpart J, Timms D, Chew M, Fung YL, Toon M, Maybauer MO, Fraser JF, Optimal management of the critically ill: anaesthesia, monitoring, data capture, and point-of-care technological practices in ovine models of critical care. Biomed Res Int, 2014():468309(2014) [pubmed]

10.1155/2014/468309;

Chemonges S, Tung JP, Fraser JF, Proteogenomics of selective susceptibility to endotoxin using circulating acute phase biomarkers and bioassay development in sheep: a review. Proteome Sci, 12(1):12(2014) [pubmed]

10.12688/F1000RESEARCH.128316.1;

10.1186/1477-5956-12-12;

curator keyword: Biological

submitter keyword: nanoLC-nanoESI-MS/MS, Peptide Spectral Library,Sheep, Proteogenomics, Circulating acellular proteome, data dependent acquisition, TripleTOF 5600+

Dr Pawel Sadowski
contact affiliation	Research Officer|Proteomics and Small Molecule Mass Spectrometry Laboratory | Central Analytical Research Facility (CARF)| Queensland University of Technology | Address: Level 6, P Block, Gardens Point Campus, 2 George St Brisbane QLD 4000
contact email	pawel.sadowski@qut.edu.au
lab head
Saul Chemonges
contact affiliation	School of Veterinary Science, The University of Queensland, Australia
contact email	s_chemonges@yahoo.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD005002

PRIDE project URI

• PRIDE
  1. PXD005002
     1. Label: PRIDE project
     2. Name: Towards a comprehensive targeted proteogenomic assay repository for the liquid fraction of sheep blood