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PXD005002

PXD005002 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTowards a comprehensive targeted proteogenomic assay repository for the liquid fraction of sheep blood
DescriptionThis dataset presents the results of protein sample preparation strategies for enriching a novel encyclopaedic peptide spectral library using the liquid fraction of sheep blood. The method used showed the capability of simultaneously detecting hundreds of key proteins in the liquid fraction of blood (circulating acellular proteome) using sheep as a model by: a) Prototyping of the circulating acellular proteome of healthy sheep using serum b) A comprehensive analysis of fractions of acetone precipitated sheep serum and plasma; c) A comprehensive analysis of fractions of partial organic precipitation of sheep serum and plasma proteins using acetonitrile; d) Combinatorial peptide ligand library protein enrichment of sheep serum and plasma; e) Off-gel fractionation of serum proteins; f) Extraction of protein data from ex-diagnostic sheep serum; g) Derivation of peptide data from serum and plasma of endotoxin treated sheep.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_13:28:30.590.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD005002
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSaul Chemonges
SpeciesList scientific name: Ovis aries; NCBI TaxID: 9940;
ModificationListGln->pyro-Glu; Glu->pyro-Glu; Oxidation; Carbamidomethyl; Deamidated
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-09-20 02:24:32ID requested
12024-10-07 13:28:33announced
Publication List
Chemonges S, Shekar K, Tung JP, Dunster KR, Diab S, Platts D, Watts RP, Gregory SD, Foley S, Simonova G, McDonald C, Hayes R, Bellpart J, Timms D, Chew M, Fung YL, Toon M, Maybauer MO, Fraser JF, Optimal management of the critically ill: anaesthesia, monitoring, data capture, and point-of-care technological practices in ovine models of critical care. Biomed Res Int, 2014():468309(2014) [pubmed]
10.1155/2014/468309;
Chemonges S, Tung JP, Fraser JF, Proteogenomics of selective susceptibility to endotoxin using circulating acute phase biomarkers and bioassay development in sheep: a review. Proteome Sci, 12(1):12(2014) [pubmed]
10.12688/F1000RESEARCH.128316.1;
10.1186/1477-5956-12-12;
Keyword List
curator keyword: Biological
submitter keyword: nanoLC-nanoESI-MS/MS, Peptide Spectral Library,Sheep, Proteogenomics, Circulating acellular proteome, data dependent acquisition, TripleTOF 5600+
Contact List
Dr Pawel Sadowski
contact affiliationResearch Officer|Proteomics and Small Molecule Mass Spectrometry Laboratory | Central Analytical Research Facility (CARF)| Queensland University of Technology | Address: Level 6, P Block, Gardens Point Campus, 2 George St Brisbane QLD 4000
contact emailpawel.sadowski@qut.edu.au
lab head
Saul Chemonges
contact affiliationSchool of Veterinary Science, The University of Queensland, Australia
contact emails_chemonges@yahoo.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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