PXD004994

PXD004994 is an original dataset announced via ProteomeXchange.

Title	Rice pollen plasma membrane iTRAQ
Description	The coordination of pollen tube (PT) growth, guidance and timely growth arrest and rupture mediated by PT-pistil interaction is crucial for the PT to transport sperm cells into ovules for double fertilization. The plasma membrane (PM) represents an important interface for cell–cell interaction, and PM proteins of PTs are pioneers for mediating PT integrity and interaction with pistils. Thus, understanding the mechanisms underlying these events is important for proteomics. Using the efficient aqueous polymer two-phase system and alkali buffer treatment, we prepared high-purity PM from mature and germinated pollen of rice. We used iTRAQ quantitative proteomic methods and identified 1,121 PM-related proteins (PMrPs) (matched to 899 loci); 192 showed differential expression in the two pollen cell types, 119 up- and 73 down-regulated during germination. The PMrP and differentially expressed PMrP sets all showed a functional skew toward signal transduction, transporters, wall remodeling/metabolism and membrane trafficking. Their genomic loci had strong chromosome bias. We found 37 receptor-like kinases (RLKs) from 8 kinase subfamilies and 209 transporters involved in flux of diversified ions and metabolites. In combination with the rice pollen transcriptome data, we revealed that in general, the protein expression of these PMrPs disagreed with their mRNA expression, with inconsistent mRNA expression for 74% of differentially expressed PMrPs. This study, for the first time, identified genome-wide pollen PMrPs, and provided insights into the membrane profile of receptor-like kinases and transporters important for pollen tube growth and interaction with pistils. These pollen PMrPs and their mRNAs showed discordant expression. This work provides novel resource and knowledge to further dissect mechanisms by which pollen or the PT controls PMrP abundance and monitors interactions and ion and metabolite exchanges with female cells in rice.
HostingRepository	PRIDE
AnnounceDate	2017-01-16
AnnouncementXML	Submission_2017-01-16_15:03:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ning Yang
SpeciesList	scientific name: Rice(O. sativa); NCBI TaxID: 4530;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-19 02:59:31	ID requested
⏵ 1	2017-01-16 15:03:41	announced

Yang N, Wang T, Comparative proteomic analysis reveals a dynamic pollen plasma membrane protein map and the membrane landscape of receptor-like kinases and transporters important for pollen tube growth and interaction with pistils in rice. BMC Plant Biol, 17(1):2(2017) [pubmed]

curator keyword: Biological

submitter keyword: pollen, plasma membrane, iTRAQ

Ning Yang
contact affiliation	Institute of Botany, Chinese Academy of Sciences
contact email	yangning07@mails.ucas.ac.cn
lab head
Ning Yang
contact affiliation	IBCAS
contact email	yangning07@mails.ucas.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004994
     1. Label: PRIDE project
     2. Name: Rice pollen plasma membrane iTRAQ