PXD004988

PXD004988 is an original dataset announced via ProteomeXchange.

Title	Grape ASR-silencing sways nuclear proteome and histone marks
Description	The biological roles of ASRs (Abscisic acid, Stress, Ripening proteins) involved in certain crucial switches of plant development (grain and pollen germination, senescence, fruit ripening), and responses to environmental cues (drought, salinity, cold, nutrient depletion, fungus infection, heavy metal toxicity) remain still elusive. The functional duality of ASRs, as chaperones for direct protection of biological macromolecules, and as transcriptional regulators of gene expression, needs in-depth understanding of the underlying mechanisms. To further elucidate the biological functions of ASRs a quantitative proteomic analysis (iTRAQ) was performed comparing the nuclear proteins of grapevine embryogenic 41B cells: wild-type (WT) and genetically modified VvMSA-RNAi cells, silenced for grape ASR. Among all identified proteins, thirty-one were differentially expressed in WT and VvMSA-silenced cells and may be classified into five functional groups: metabolism, stress responses, ribosome biogenesis, transcription and chromatin architecture/remodeling. The impact of VvMSA silencing was also confirmed at the level of the genes encoding the differentially expressed nuclear proteins, and the revealed most pronounced effects at protein level suggest a more direct correlation to the putative biological function. Remarkably, many of the thirty-one differentially expressed proteins are supposedly involved in epigenetic events. To further corroborate this hypothesis immunoblot assay of Multiscreen type was performed using twenty-one antibodies to different post-translational modifications (PTMs) of histones H3 and H4 in both cell types, WT and VvMSA-RNAi. The subsequent ImageQuant analysis revealed marked semi-quantitative differences in most PTMs of grape histones H3 and H4, with sharp increase of H3K27ac, H3K36me3, H4K5ac and H4K12ac, as well as a strong decrease of H3K27me3 in VvMSA-RNAi cells. These findings provide new insights on a plausible role of VvMSA as an ASR protein involved in epigenetic mechanisms
HostingRepository	PRIDE
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-02-22_04:13:19.104.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004988
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bernd Roschitzki
SpeciesList	scientific name: Vitis vinifera (Grape); NCBI TaxID: 29760;
ModificationList	Methylthio; Deamidated; Oxidation; iTRAQ8plex
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-16 05:17:27	ID requested
⏵ 1	2022-02-22 04:13:19	announced

Atanassov H, Parrilla J, Artault C, Verbeke J, Schneider T, Grossmann J, Roschitzki B, Atanassova R, Grape ASR-Silencing Sways Nuclear Proteome, Histone Marks and Interplay of Intrinsically Disordered Proteins. Int J Mol Sci, 23(3):(2022) [pubmed]

ProteomeXchange project tag: PRIME-XS Project

curator keyword: Biological

submitter keyword: iTRAQ, LC-MS/MS, grape

Rossitza Atanassova
contact affiliation	Université de Poitiers, UMR CNRS 7267 Écologie & Biologie des Interactions, Équipe Sucres & Echanges Végétaux-Environnement, 3 rue Jacques Fort, 86073 Poitiers, France
contact email	rossitza.atanassova@univ-poitiers.fr
lab head
Bernd Roschitzki
contact affiliation	University of Zurich
contact email	bernd.roschitzki@fgcz.uzh.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD004988

PRIDE project URI

• PRIDE
  1. PXD004988
     1. Label: PRIDE project
     2. Name: Grape ASR-silencing sways nuclear proteome and histone marks