PXD004983

PXD004983 is an original dataset announced via ProteomeXchange.

Title	AP-MS analysis of m7GTP-binding proteins in Murine norovirus infected BV-2 cells (v2)
Description	SILAC-labelling analysis of murine BV-2 cells infected at high multiplicity of infection (10 TCID50/cell) and harvested at 4h or 9h post-infection. Lysates were then suject to m7GTP-sepharose affinity purification to enrich for translation initiation factors. As norovirus translation uses a VPg protein covalently linked to the 5' of its RNAs for initiation factor recruitment it was hypothesised that norovirus infection could modify initiation factor complexes. A paired dataset investigates the effects of MNV infection on total protein levels in infected cells. This dataset is a Maxquant reanalysis of data previously submitted under PXD004019
HostingRepository	PRIDE
AnnounceDate	2017-01-17
AnnouncementXML	Submission_2017-01-17_13:36:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Edward Emmott
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	6x(13)C labeled residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-16 02:27:04	ID requested
⏵ 1	2017-01-17 13:36:21	announced

Emmott E, Sorgeloos F, Caddy SL, Vashist S, Sosnovtsev S, Lloyd R, Heesom K, Locker N, Goodfellow I, Norovirus-Mediated Modification of the Translational Landscape via Virus and Host-Induced Cleavage of Translation Initiation Factors. Mol Cell Proteomics, 16(4 suppl 1):S215-S229(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: SILAC, m7GTP, translation, initiation, norovirus, MNV

Ian Goodfellow
contact affiliation	University of Cambridge, Division of Virology, Department of Pathology.
contact email	ig299@cam.ac.uk
lab head
Edward Emmott
contact affiliation	University of Cambridge
contact email	ee273@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD004983

PRIDE project URI

• PRIDE
  1. PXD004983
     1. Label: PRIDE project
     2. Name: AP-MS analysis of m7GTP-binding proteins in Murine norovirus infected BV-2 cells (v2)