PXD004975

PXD004975 is an original dataset announced via ProteomeXchange.

Title	Effects of Iron Deficiency and Risperidone treatment on the rat frontal cortex
Description	Prenatal iron deficiency (pID) has been described to increase the risk for neurodevelopmental disorders such as autism and schizophrenia; however, the precise molecular mechanisms are still unknown. Here, we utilized high throughput mass spectrometry to examine the proteomic effects of pID in adulthood on the rat frontal cortex area (FCA). In addition, the FCA proteome was examined in adulthood following risperidone treatment in adolescence to see if these effects could be prevented. We identified 1501 proteins of which 100 were significantly differentially expressed in the FCA at post-natal day 90. Pathway Analysis of proteins affected by pID revealed changes in metabolic processes, including the tricyclic acid cycle, mitochondrial dysfunction, and P13K/Akt signaling. Interestingly, most of these protein changes were not present in the adult pID offspring who received risperidone in adolescence. Behavioral testing of pID rats demonstrated social impairment and poor performance during novelty-induced exploration in pID animals in line with a abnormal neurodevelopmental phenotype. Considering the link between prenatal iron deficiency and several neurodevelopmental disorders such as autism and schizophrenia these presented results bring new perspectives to understand the role of iron in metabolic pathways and provide novel biomarkers for future studies of prenatal iron deficiency.
HostingRepository	PRIDE
AnnounceDate	2018-07-05
AnnouncementXML	Submission_2018-07-05_05:40:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Melanie Foecking
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	carbamoylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-15 03:26:44	ID requested
⏵ 1	2018-07-05 05:40:31	announced

Farrelly L, Rosato-Siri MV, F, ö, cking M, Codagnone M, Reines A, Dicker P, Wynne K, Farrell M, Cannon M, Cagney G, Pasquini JM, Cotter DR, A Proteomic Analysis. Proteomics, 17(17-18):(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: rat, iron defiency, risperidone, LC MSMS

David Cotter
contact affiliation	Royal College of Surgeons in Ireland, Department of Psychiatry
contact email	drcotter@rcsi.ie
lab head
Melanie Foecking
contact affiliation	Royal College of Surgeons in Ireland
contact email	mfocking@rcsi.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004975
     1. Label: PRIDE project
     2. Name: Effects of Iron Deficiency and Risperidone treatment on the rat frontal cortex