PXD004974

PXD004974 is an original dataset announced via ProteomeXchange.

Title	Proteomic analyses reveal metabolic responses to 3-hydroxypropionic acid synthesized internally in Synechocystis sp. PCC 6803
Description	Using an engineered 3-HP producing SM strain, in this project, the cellular responses to 3-HP internally produced were first determined using a quantitative iTRAQ-LC-MS/MS proteomics approach. A total of 2,264 unique proteins were identified, which represented about 63% of all predicted protein in Synechocystis in the proteomic analysis of the 3-HP producing strain SM and wild type. Among all identified proteins, 204 proteins were found up- regulated and 123 proteins were found down-regulated, respectively. The proteins related to oxidative phosphorylation, photosynthesis, ribosome, central carbon metabolism, two-component systems and ABC-type transporters were up-regulated. The results suggested that the supply of ATP and NADPH was increased significantly, and the precursor malonyl-CoA and acetyl-CoA may also be supplemented when 3-HP was produced at a high level in Synechocystis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:10:05.568.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yunpeng Wang
SpeciesList	scientific name: Synechococcus cyanophage syn; NCBI TaxID: 382352;
ModificationList	No PTMs are included in the dataset
Instrument	4700 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-15 02:47:07	ID requested
1	2016-09-26 05:34:44	announced
2	2016-10-24 00:58:33	announced	Updated publication reference for PubMed record(s): 27757169.
⏵ 3	2024-10-22 04:10:07	announced	2024-10-22: Updated project metadata.

Wang Y, Chen L, Zhang W, sp. PCC 6803. Biotechnol Biofuels, 9():209(2016) [pubmed]

curator keyword: Biological

submitter keyword: Synechocystis,3-HP, Proteomics

Weiwen Zhang
contact affiliation	Tianjin University
contact email	wwzhang22@outlook.com
lab head
Yunpeng Wang
contact affiliation	Tianjin University
contact email	wangyunpeng4321@sina.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004974
     1. Label: PRIDE project
     2. Name: Proteomic analyses reveal metabolic responses to 3-hydroxypropionic acid synthesized internally in Synechocystis sp. PCC 6803