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PXD004969 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMolecular data integration reveals Growth Hormone Receptor as an alternative candidate oncogenic signaling pathway in glioblastoma
DescriptionOur laboratory has identified the Growth Hormone Receptor (GHR) as a candidate oncogenic pathway involved in GBM oncogenicity. In order to study further the role of GHR in GBM, we have generated GHR overexpressing patient-derived cell lines (PDCL) from PDCL established by GlioTEx team (Institut du Cerveau et de la Moelle, Paris, France). An analysis of the global proteome of each variant was then undertaken. The wild-type (GHR WT) and constitutively activated (GHR CA) GHR expression vectors were generous gifts from Mike Waters and Peter Brooks (University of Queensland, Australia). The constitutively activated GHR vector was generated by fusing the transmembrane and cytoplasmic domains of rabbit GHR (to ensure the absence of human GH stimulation) to Jun zippers in order to achieve GH-independent forced dimerization in absence of the extracellular domain. The control GFP vector was obtained from Addgene . These vectors were then inserted in lentiviral vectors. Cells were transduced with multiplicity of infection of 20 and transduced cells were selected with puromycin.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGuillaume Arras
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-09-14 04:00:55ID requested
12022-06-23 04:26:28announced
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biomedical
submitter keyword: Glioblastoma, GHR, SOCS2, EGFR, cellular movement, gains of function, proteomic, expression array
Contact List
Damarys Loew
contact affiliationLaboratoire de Spectrométrie de Masse Protéomique (LSMP) Institut Curie
contact emailDamarys.Loew@curie.fr
lab head
Guillaume Arras
contact affiliationInstitut Curie
contact emailguillaume.arras@curie.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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